AB109496

重组Anti-HEC1/HEC抗体[EPR5342]

Anti-HEC1/HEC antibody [EPR5342]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal HEC1/HEC antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

HEC, HEC1, KNTC2, NDC80, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, HsHec1

1 Images

Unknown

Western blot - Anti-HEC1/HEC antibody [EPR5342] (AB109496)

All lanes:

Western blot - Anti-HEC1/HEC antibody [EPR5342] (ab109496) at 1/10000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

NCCIT lysate at 10 µg

Lane 4:

Human spleen lysate at 10 µg

Predicted band size: 74 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-HEC1/HEC antibody [EPR5342] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5342

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HEC1 also known as NDC80 or HEC is an important component of the kinetochore a protein complex importantly involved in chromosome segregation during cell division. It is often referred to as HEC-1 with a molecular mass of approximately 80 kDa. HEC1 is expressed ubiquitously in proliferating cells and it plays an important role in the accurate alignment and segregation of chromosomes during mitosis. The protein is found at centromeres where it is essential for proper attachment of microtubules.

Biological function summary

In the context of cell division HEC1 functions as part of the NDC80 complex a critical ensemble of four proteins that include NUF2 SPC24 and SPC25. This complex is vital for microtubule binding and kinetochore function. HEC1 as a subunit of NDC80 ensures kinetic stability and maintains the structural integrity of the kinetochore during the cell cycle. Its function is necessary for accurate chromosome alignment on the metaphase plate which prevents missegregation and aneuploidy.

Pathways

HEC1 is actively involved in the spindle assembly checkpoint (SAC) a safety mechanism that prevents anaphase onset until all chromosomes are properly aligned. This pathway is important in regulating cell cycle progression and preventing genomic instability. HEC1 also interacts with proteins like BUB1 and MAD2 which are integral to the SAC pathway. HEC1’s associations highlight its role in maintaining mitotic accuracy and integrity in dividing cells.

HEC1 has a significant connection to cancer. Aberrant expression or malfunction of HEC1 contributes to chromosomal instability a hallmark of cancer progression. Its interactions with oncogenes and tumor suppressor proteins like TP53 can exacerbate this instability leading to unregulated cell division and tumor growth. Additionally HEC1's role in the cell cycle and proliferation links it to disorders like aneuploidies where chromosome number errors occur further implicating its importance in maintaining chromosomal balance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12351790, PubMed : 14654001, PubMed : 14699129, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 16732327, PubMed : 30409912, PubMed : 9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592, PubMed : 30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed : 23891108, PubMed : 25743205).

See full target information NDC80

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Neuroendocrinology 115:618-631 PubMed40188826

2025

Upregulation of SSTR2 Expression and Radioligand Binding of [18F]SiTATE in Neuroendocrine Tumour Cells with Combined Inhibition of Class I HDACs and LSD1.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph J Auernhammer,Kathrin Zitzmann,Simon Lindner,Harun Ilhan,Peter Bartenstein,Umberto Maccio,Michael Orth,Lea Peischer,Julian Maurer,Gerald Spoettl,Katharina Wang,Christine Spitzweg,Alessa Fischer,Constanze Hantel,Ashley B Grossman,Felix Beuschlein,Karel Pacak,Svenja Nölting

Experimental and therapeutic medicine 26:480 PubMed37745040

2023

Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Luo,Yumei Zhong,Yanding Guo,Jingwei Nie,Yimei Xu,Haiyan Zhou

View all publications

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重组Anti-HEC1/HEC抗体[EPR5342]