Anti-HEATR6抗体(ab122131)
Key features and details
- Rabbit polyclonal to HEATR6
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HEATR6抗体 -
描述
兔多克隆抗体to HEATR6 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 1052-1175 of Human HEATR6.
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阳性对照
- RT-4 and U-251 MG cell lysates; Human kidney tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122131于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Amplification-dependent oncogene. -
组织特异性
Amplified in breast cancer cell lines MCF7 and BT474. -
序列相似性
Contains 4 HEAT repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 63897 Human
- SwissProt: Q6AI08 Human
- Unigene: 591183 Human
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别名
- Amplified in breast cancer protein 1 antibody
- HEAT repeat-containing protein 6 antibody
- HEAT6_HUMAN antibody
- HEATR6 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (ab122131)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-HEATR6 antibody (ab122131)All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (ab122131)ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab122131 尚未被引用在任何文献中。