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AB241610

Anti-HEATR1抗体

Anti-HEATR1 antibody

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(3 Publications)

Rabbit Polyclonal HEATR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HEATR1 aa 2050 to C-terminus.

查看别名

BAP28, UTP10, HEATR1, HEAT repeat-containing protein 1, Protein BAP28, U3 small nucleolar RNA-associated protein 10 homolog

2 Images
Immunoprecipitation - Anti-HEATR1 antibody (AB241610)
  • IP

Supplier Data

Immunoprecipitation - Anti-HEATR1 antibody (AB241610)

HEATR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241610 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241610 at 0.4 μg/ml.

Lane 1 : ab241610 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HEATR1 antibody (ab241610)

Predicted band size: 242 kDa

false

Western blot - Anti-HEATR1 antibody (AB241610)
  • WB

Supplier Data

Western blot - Anti-HEATR1 antibody (AB241610)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HEATR1 antibody (ab241610) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Predicted band size: 242 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human HEATR1 aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H583

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HEATR1 also known as HEAT repeat-containing protein 1 is a cytoplasmic protein with a molecular mass of approximately 166 kDa. The protein features several HEAT repeats which are tandemly arranged alpha-helical motifs. HEATR1 expresses in various tissues with high levels detected in the testes and lower levels in brain and lungs. The protein plays a role in cellular processes like protein trafficking and folding contributing to ribosome biogenesis and maintenance of cell cycle progression.
Biological function summary

HEATR1 influences pathways involved in the assembly and maturation of the ribosomal machinery. It forms part of the ribosome assembly complex associating with other ribosome biogenesis factors to facilitate ribosome production. The involvement of HEATR1 in the assembly complex helps ensure the ribosomes are correctly formed and functional impacting protein synthesis efficiency.

Pathways

HEATR1 interacts with key regulators in the ribosome biogenesis pathway and is linked to cell cycle regulation. It connects to proteins such as NOP14 and WDR25 within these pathways highlighting its importance in ribosome assembly and cellular division. The accurate functioning of these pathways is critical for cell growth and proliferation affecting overall cellular health and metabolism.

HEATR1 may be related to ribosomopathies and certain cancers where defects in ribosome biogenesis or aberrant protein synthesis play a role. The protein’s interactions with NOP14 suggest potential connections with disorders in ribosome production which may lead to proliferation issues observed in malignancies. Understanding the role of HEATR1 can offer insights into the molecular mechanisms behind these conditions aiding in the development of new therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Ribosome biogenesis factor; required for recruitment of Myc to nucleoli (PubMed : 38225354). Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I (PubMed : 17699751). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Involved in neuronal-lineage cell proliferation (PubMed : 38225354).
See full target information HEATR1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 614:160-167 PubMed36697828

2023

Visualization of translation and protein biogenesis at the ER membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Max Gemmer,Marten L Chaillet,Joyce van Loenhout,Rodrigo Cuevas Arenas,Dimitrios Vismpas,Mariska Gröllers-Mulderij,Fujiet A Koh,Pascal Albanese,Richard A Scheltema,Stuart C Howes,Abhay Kotecha,Juliette Fedry,Friedrich Förster

Biochemical and biophysical research communications 557:294-301 PubMed33894417

2021

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Nakamura,Yoshito Kakihara,Akinori Funayama,Kenta Haga,Toshihiko Mikami,Daiki Kobayashi,Yutaka Yoshida,Kenji Izumi,Tadaharu Kobayashi,Makio Saeki

Journal of oncology 2020:3181596 PubMed32565799

2020

HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fang,Xu Han,Jianang Li,Tiantao Kuang,Wenhui Lou
View all publications
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