Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-HDGF抗体[EPR7898] (ab128921)

Reviews (2) Submit a question References (8)
Western blot - Anti-HDGF antibody [EPR7898] (ab128921)
  • Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7898] (ab128921)
  • Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7898] (ab128921)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)
  • Anti-HDGF antibody [EPR7898] (ab128921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7898] to HDGF
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

概述

  • 产品名称

    Anti-HDGF抗体[EPR7898]
    参阅全部 HDGF 一抗

  • 描述

    兔单克隆抗体[EPR7898] to HDGF

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human HDGF aa 100-200. The exact sequence is proprietary.

  • 阳性对照

    • HeLa, A549, SKBR-3 and ECV-304 cell lysates; Human hepatocellular carcinoma and Human colon tissues

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR7898

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab128921于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
说明
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.

靶标

  • 功能

    Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.

  • 组织特异性

    Ubiquitous.

  • 序列相似性

    Belongs to the HDGF family.
    Contains 1 PWWP domain.

  • 结构域

    The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.

  • 翻译后修饰

    Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • FLJ96580 antibody
    • HDGF antibody
    • HDGF_HUMAN antibody
    • Hepatoma derived growth factor antibody
    • Hepatoma-derived growth factor antibody
    • High mobility group protein 1 like 2 antibody
    • High mobility group protein 1-like 2 antibody
    • HMG-1L2 antibody
    • HMG1L2 antibody
    see all

图片

  • Western blot - Anti-HDGF antibody [EPR7898] (ab128921)
    Western blot - Anti-HDGF antibody [EPR7898] (ab128921)
    All lanes : Anti-HDGF antibody [EPR7898] (ab128921) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : SKBR-3 cell lysate
    Lane 4 : ECV-304 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7898] (ab128921)
    Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7898] (ab128921)

    ab128921 staining HDGF in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7898] (ab128921)
    Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7898] (ab128921)

    Overlay histogram showing HeLa cells stained with ab128921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)

    ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human hepatocellular carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7898] (ab128921)

    ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-HDGF antibody [EPR7898] (ab128921)
    Anti-HDGF antibody [EPR7898] (ab128921)

文献 (8)

发表研究结果有使用 ab128921？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab128921 被引用在 8 文献中.

  • Voelkl K  et al. Neuroprotective effects of hepatoma-derived growth factor in models of Huntington's disease. Life Sci Alliance 6:N/A (2023). PubMed: 37580082
  • Bao J  et al. Long noncoding RNA LINC00649 functions as a microRNA‑432‑5p sponge to facilitate tumourigenesis in colorectal cancer by upregulating HDGF. Mol Med Rep 25:N/A (2022). PubMed: 35088877
  • Tian B  et al. Long Noncoding RNA LIPE-AS1 Drives Prostate Cancer Progression by Functioning as a Competing Endogenous RNA for microRNA-654-3p and Thereby Upregulating Hepatoma-Derived Growth Factor. Urol Int 105:875-890 (2021). PubMed: 34233322
  • Situ J  et al. MicroRNA-939 Directly Targets HDGF to Inhibit the Aggressiveness of Prostate Cancer via Deactivation of the WNT/ß-Catenin Pathway. Onco Targets Ther 13:4257-4270 (2020). PubMed: 32547060
  • Zhao M  et al. Long intergenic non-coding RNA LINC01232 contributes to esophageal squamous cell carcinoma progression by sequestering microRNA-654-3p and consequently promoting hepatoma-derived growth factor expression. Int J Mol Med 46:2007-2018 (2020). PubMed: 33125097
  • Gao W  et al. Long non-coding RNA MKLN1-AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR-654-3p, thereby promoting hepatoma-derived growth factor expression. Int J Mol Med 46:1743-1754 (2020). PubMed: 33000222
  • Zheng Y  et al. Long non-coding RNA AGAP2-AS1 promotes the proliferation of glioma cells by sponging miR-15a/b-5p to upregulate the expression of HDGF and activating Wnt/ß-catenin signaling pathway. Int J Biol Macromol 128:521-530 (2019). PubMed: 30684575
  • Dou X  et al. Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor. Front Pharmacol 10:1700 (2019). PubMed: 32082174

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HDGF antibody [EPR7898]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human fetal membranes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
Yes - 0.5% Tween
Specification
Human fetal membranes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katharina Gegenschatz

Verified customer

提交于 Apr 20 2022

Western blot abreview for Anti-HDGF antibody [EPR7898]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Treatment
treated with siRNA against Luciferase (Luc) and HDGF (si HDGF-1 and 2) for 72 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 15 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com