Anti-HDGF抗体[26C4a] (ab58644)
![Western blot - Anti-HDGF antibody [26C4a] (ab58644)](https://www.abcam.cn/ps/products/58/ab58644/Images/ab58644.gif "Western blot - Anti-HDGF antibody [26C4a] (ab58644)")
Key features and details
- Mouse monoclonal [26C4a] to HDGF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-HDGF抗体[26C4a]
参阅全部 HDGF 一抗 -
描述
小鼠单克隆抗体[26C4a] to HDGF -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment of human HDGF, from an internal sequence.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
26C4a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58644于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa). |
靶标
-
功能
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the HDGF family.
Contains 1 PWWP domain. -
结构域
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured. -
翻译后修饰
Sumoylated by SUMO1. Sumoylation prevents binding to chromatin. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
别名
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
see all
图片
-
Western blot - Anti-HDGF antibody [26C4a] (ab58644)Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein
at 25 µg
Secondary
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The immunogen shown in the WB is tagged.
数据表及文件
-
Datasheet download
文献 (3)
ab58644 被引用在 3 文献中.
- Hong YG et al. MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF. Biomed J 43:163-173 (2020). PubMed: 32418767
- Leich E et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P ; Human . PubMed: 21960592
- Maschietto M et al. Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Cell Death Dis 2:e224 (2011). PubMed: 22048167
