重组Anti-HDAC6抗体[EPR1698(2)] (ab133493)
![Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-448917-anti-hdac6-antibody-epr16982-western-blot-hela-lysates.jpg "Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1698(2)] to HDAC6
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human, African green monkey
Related conjugates and formulations
概述
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产品名称
Anti-HDAC6抗体[EPR1698(2)]
参阅全部 HDAC6 一抗 -
描述
兔单克隆抗体[EPR1698(2)] to HDAC6 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Monkey
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免疫原
Synthetic peptide within Human HDAC6 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UBN7 -
阳性对照
- WB: HAP1, HeLa, Jurkat, and COS-1 cell lysates. Flow Cyt (intra): K562. IHC-P: Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR1698(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133493于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
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| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/200. |
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WB
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
翻译后修饰
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
细胞定位
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
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别名
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
图片
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Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HDAC6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab133493 was shown to react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line was used. Wild-type HeLa and HDAC6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-272313-anti-hdac6-antibody-epr16982-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-147152-anti-hdac6-antibody-epr16982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HDAC6 with ab133493 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-252491-anti-hdac6-antibody-epr16982-western-blot.jpg)
Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)Lanes 1-2 : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lanes 3-4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution
Lanes 1 & 3 & 5 : Wild-type HAP1 cell lysate
Lanes 2 & 4 & 6 : HDAC6 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 131 kDaLanes 1 and 2: Green signal from target – ab133493 observed at 160 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signalab133493 was shown to specifically react with HDAC6 when HDAC6 knockout samples were used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. ab133493 and ab8226 (loading control to beta actin) were diluted 1/10 000 and 1/1000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-147153-anti-hdac6-antibody-epr16982-western-blot.jpg)
Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : COS-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted? -
![Anti-HDAC6 antibody [EPR1698(2)] (ab133493)](https://www.abcam.cn/ps/products/133/ab133493/Images/ab133493-6-benefits-of-recombinant-antibodies.png)
Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab133493 被引用在 14 文献中.
- Yang D et al. Pharmacological Targeting of Bcl-2 Induces Caspase 3-Mediated Cleavage of HDAC6 and Regulates the Autophagy Process in Colorectal Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 37047634
- Zhao T et al. Qi-Dong-Huo-Xue-Yin balances the immune microenvironment to protect against LPS induced acute lung injury. Front Pharmacol 14:1200058 (2023). PubMed: 37292149
- Nie Q et al. Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel. Mol Med Rep 28:N/A (2023). PubMed: 37681444
- Shin HS et al. Histone Deacetylase as a Valuable Predictive Biomarker and Therapeutic Target in Immunotherapy for Non-Small Cell Lung Cancer. Cancer Res Treat 54:458-468 (2022). PubMed: 34517693
- Yuan B et al. Patient-derived organoids for personalized gallbladder cancer modelling and drug screening. Clin Transl Med 12:e678 (2022). PubMed: 35075805