Anti-HDAC5 (phospho S498)抗体(ab47283)
Key features and details
- Rabbit polyclonal to HDAC5 (phospho S498)
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HDAC5 (phospho S498)抗体
参阅全部 HDAC5 一抗 -
描述
兔多克隆抗体to HDAC5 (phospho S498) -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
Database link: Q9UQL6 -
阳性对照
- NIH/3T3 cell extracts; Breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47283于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
结构域
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻译后修饰
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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数据库链接
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Entrez Gene: 84580 Rat
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
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别名
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
图片
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Western blot - Anti-HDAC5 (phospho S498) antibody (ab47283)All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution
Lane 1 : Extracts from NIH/ 3T3 cells
Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)
Predicted band size: 122 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 (phospho S498) antibody (ab47283)ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab47283 被引用在 14 文献中.
- Truong V et al. Angiotensin II-induced histone deacetylase 5 phosphorylation, nuclear export, and Egr-1 expression are mediated by Akt pathway in A10 vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 320:H1543-H1554 (2021). PubMed: 33606583
- Pietruczuk P et al. Protein kinase B mediates insulin-like growth factor 1-induced phosphorylation and nuclear export of histone deacetylase 5 via NADPH oxidase 4 activation in vascular smooth muscle cells. J Cell Physiol N/A:N/A (2019). PubMed: 30793765
- Tóth AD et al. Inflammation leads through PGE/EP3 signaling to HDAC5/MEF2-dependent transcription in cardiac myocytes. EMBO Mol Med 10:N/A (2018). PubMed: 29907596
- Meng ZX et al. Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis. Mol Cell 66:332-344.e4 (2017). PubMed: 28475869
- Weeks KL et al. ß-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55a-PP2A-Mediated Dephosphorylation. J Am Heart Assoc 6:N/A (2017). PubMed: 28343149