Anti-HDAC5抗体(ab55403)
Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HDAC5抗体
参阅全部 HDAC5 一抗 -
描述
兔多克隆抗体to HDAC5 -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, IHC-FoFr, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
Database link: Q9UQL6 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab55403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55403于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23469282
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21526203
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| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23469282 |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 21526203 |
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WB
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
结构域
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻译后修饰
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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数据库链接
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
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别名
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (ab55403)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
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Western blot - Anti-HDAC5 antibody (ab55403)All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution
Lane 1 : HepG2 cell extract, without immunizing peptide
Lane 2 : HepG2 cell extract, with immunizing peptide
Predicted band size: 122 kDa
Observed band size: 122 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab55403 被引用在 18 文献中.
- Lv Z et al. Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide. JCI Insight 8:N/A (2023). PubMed: 37870958
- Shen J et al. Stenotrophomonas maltophilia promotes lung adenocarcinoma progression by upregulating histone deacetylase 5. Front Microbiol 14:1121863 (2023). PubMed: 36819033
- Sun JY et al. An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction. Sci Adv 9:eade4110 (2023). PubMed: 37018396
- Jiang R et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). WB, IHC-P ; Human . PubMed: 35396379
- Duncan HF et al. The Critical Role of MMP13 in Regulating Tooth Development and Reactionary Dentinogenesis Repair Through the Wnt Signaling Pathway. Front Cell Dev Biol 10:883266 (2022). PubMed: 35531096