Anti-HDAC4 (phospho S632)抗体(ab39408)
Key features and details
- Rabbit polyclonal to HDAC4 (phospho S632)
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-HDAC4 (phospho S632)抗体
参阅全部 HDAC4 一抗 -
描述
兔多克隆抗体to HDAC4 (phospho S632) -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human HDAC4 aa 550-650 (phospho S632).
Database link: P56524 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39408于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 119 kDa.
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| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 119 kDa. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. -
组织特异性
Ubiquitous. -
疾病相关
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. -
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
结构域
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻译后修饰
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
别名
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
图片
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Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)
Predicted band size: 119 kDa -
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit. -
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)Image courtesy of an anonymous Abreview.All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Lysate from murine heart tissue 1
Lane 2 : Lysate from murine heart tissue 2
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab39408 被引用在 14 文献中.
- Lax A et al. Silencing of microRNA-106b-5p prevents doxorubicin-mediated cardiotoxicity through modulation of the PR55α/YY1/sST2 signaling axis. Mol Ther Nucleic Acids 32:704-720 (2023). PubMed: 37234747
- Li YY et al. PTHrP promotes development of mouse preimplantation embryos through the AKT/cyclin D1 pathway and nuclear translocation of HDAC4. J Cell Physiol 236:7001-7013 (2021). PubMed: 33724469
- Antunes-Correa LM et al. Effects of aerobic and inspiratory training on skeletal muscle microRNA-1 and downstream-associated pathways in patients with heart failure. J Cachexia Sarcopenia Muscle 11:89-102 (2020). PubMed: 31743617
- Schader T et al. Oxidation of HDAC4 by Nox4-derived H2O2 maintains tube formation by endothelial cells. Redox Biol 36:101669 (2020). PubMed: 32818796
- Shi C et al. Leukocyte integrin signaling regulates FOXP1 gene expression via FOXP1-IT1 long non-coding RNA-mediated IRAK1 pathway. Biochim Biophys Acta Gene Regul Mech 1862:493-508 (2019). PubMed: 30831269