Anti-HDAC2抗体[HDAC2-62] (ab12169)
![Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.cn/ps/products/12/ab12169/Images/ab12169-213428-anti-hdac2-antibody-hdac2-62-chip-grade-immunoprecipitation.jpg "Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)")
Key features and details
- Mouse monoclonal [HDAC2-62] to HDAC2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-HDAC2抗体[HDAC2-62]
参阅全部 HDAC2 一抗 -
描述
小鼠单克隆抗体[HDAC2-62] to HDAC2 -
宿主
Mouse -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
SGEKTDTKGTKSEQLSNP
with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2. -
阳性对照
- Whole cell extracts from NIH-3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HDAC2-62 -
同种型
IgG2b -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12169于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB | (3) |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
组织特异性
Widely expressed; lower levels in brain and lung. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3066 Human
- Entrez Gene: 15182 Mouse
- Omim: 605164 Human
- SwissProt: Q92769 Human
- SwissProt: P70288 Mouse
- Unigene: 3352 Human
- Unigene: 19806 Mouse
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别名
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
图片
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Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
Western Blot using Rabbit anti-HDAC2
Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
Lane 3 : Negative Control at 5 µg
All lanes : HeLa whole cell extract -
![Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.cn/ps/products/12/ab12169/Images/ab12169-213427-anti-hdac2-antibody-hdac2-62-chip-grade-western-blot.jpg)
Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml
Lane 1 : Hek293T cell Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : K562 cell Lysate
Lane 5 : Neuro-2a cell Lysate
Lane 6 : NIH-3T3 cell Lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase -
![Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.cn/ps/products/12/ab12169/Images/ab12169-19685-anti-hdac2-antibody-hdac2-62-chip-grade-western-blot.jpg)
Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)This image is courtesy of an anonymous abreview.Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
Secondary
HRP-conjugated Goat anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (77)
ab12169 被引用在 77 文献中.
- Chen Y et al. Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice. Nat Commun 14:1521 (2023). PubMed: 36934083
- Yuan W et al. Lactobacillus rhamnosus GG and butyrate supplementation in rats with bone cancer reduces mechanical allodynia and increases expression of μ-opioid receptor in the spinal cord. Front Mol Neurosci 16:1207911 (2023). PubMed: 37389091
- Jevtic Z et al. SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation. J Exp Clin Cancer Res 41:34 (2022). PubMed: 35073946
- Dufour CR et al. The mTOR chromatin-bound interactome in prostate cancer. Cell Rep 38:110534 (2022). PubMed: 35320709
- Xu 许潇依 X et al. CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. J Neurosci 42:4594-604 (2022). PubMed: 35501151