Anti-HDAC2 抗体

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody (AB7029)

HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/600 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minute

This image is courtesy of Michael Mancini, Baylor College of Medicine

• IP

Supplier Data

Immunoprecipitation - Anti-HDAC2 antibody (AB7029)

Immunoprecipitation analysis of HeLa whole cell lysates, ab7029 was used to precipitate HDAC3 from solution.

Lane 1:

Immunoprecipitation - Anti-HDAC2 antibody (ab7029) at 5 µL

Lane 2:

Immunoprecipitation - Anti-HDAC2 antibody (ab7029) at 10 µL

Lane 3:

Negative Control

All lanes:

HeLa whole cell lysate.

Predicted band size: 55 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-HDAC2 antibody (AB7029)

HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7029.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : Band : 60ka : HDAC2; 55kDa; Heavy Chain.

All lanes:

Immunoprecipitation - Anti-HDAC2 antibody (ab7029)

Predicted band size: 55 kDa

false