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使用敲除细胞株进行验证

Anti-HDAC2抗体[3F3] (ab51832)

Reviews (8)Q&A (2)References (27)
Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [3F3] (ab51832)
  • Western blot - Anti-HDAC2 antibody [3F3] (ab51832)
  • Flow Cytometry (Intracellular) - Anti-HDAC2 antibody [3F3] (ab51832)
  • Western blot - Anti-HDAC2 antibody [3F3] (ab51832)

Key features and details

  • Mouse monoclonal [3F3] to HDAC2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

概述

  • 产品名称

    Anti-HDAC2抗体[3F3]
    参阅全部 HDAC2 一抗

  • 描述

    小鼠单克隆抗体[3F3] to HDAC2

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow

  • 免疫原

    Synthetic peptide corresponding to Human HDAC2 aa 450-550.

  • 阳性对照

    • Nuclear extract of HeLa cells and HAP1 whole cell lysate . IF/ICC: MCF7 cell line.

  • 常规说明

    This monoclonal antibody to HDAC2 has been knockout validated in Western blot. The expected band for HDAC2 was observed in wild type cells and the band was not seen in HDAC2 knockout cells.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51832于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (4)
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF (1)
1/25 - 1/100.
说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF
1/25 - 1/100.

靶标

  • 功能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.

  • 组织特异性

    Widely expressed; lower levels in brain and lung.

  • 序列相似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • 翻译后修饰

    S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [3F3] (ab51832)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [3F3] (ab51832)
    ab51832 staining HDAC2 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab51832 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
  • Western blot - Anti-HDAC2 antibody [3F3] (ab51832)
    Western blot - Anti-HDAC2 antibody [3F3] (ab51832)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HDAC2 knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: A431 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51832 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab51832 detected the expected band for HDAC2 in wild-type HAP1 cells and the band was not seen in HDAC2 knockout HAP1 cells. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. ab51832 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-HDAC2 antibody [3F3] (ab51832)
    Flow Cytometry (Intracellular) - Anti-HDAC2 antibody [3F3] (ab51832)
    Overlay histogram showing HeLa cells stained with ab51832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51832, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-HDAC2 antibody [3F3] (ab51832)
    Western blot - Anti-HDAC2 antibody [3F3] (ab51832)
    All lanes : Anti-HDAC2 antibody [3F3] (ab51832) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab51832 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

文献 (27)

发表研究结果有使用 ab51832？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab51832 被引用在 27 文献中.

  • Rivera C  et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. Nat Commun 13:1550 (2022). PubMed: 35322029
  • Noches V  et al. Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus. Biochem Biophys Rep 25:100889 (2021). PubMed: 33426312
  • Li Z  et al. Gut microbiota regulate cognitive deficits and amyloid deposition in a model of Alzheimer's disease. J Neurochem 155:448-461 (2020). PubMed: 32319677
  • Feng Q  et al. MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. Autophagy N/A:1-18 (2019). PubMed: 31223056
  • Hou X  et al. Suppression of HDAC2 in Spinal Cord Alleviates Mechanical Hyperalgesia and Restores KCC2 Expression in a Rat Model of Bone Cancer Pain. Neuroscience 377:138-149 (2018). WB, IHC-P ; Rat . PubMed: 29482000
View all Publications for this product

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1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-HDAC2 antibody [3F3]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 13 2019

Western blot abreview for Anti-HDAC2 antibody [3F3]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Raji, RcK8)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Raji, RcK8
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Xiao Qing Lu

Verified customer

提交于 Jan 25 2016

Western blot abreview for Anti-HDAC2 antibody [3F3]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Schwann cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
200 µg
Specification
Schwann cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Tom Hu

Verified customer

提交于 Mar 30 2012

ChIP abreview for Anti-HDAC2 antibody [3F3]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - whole (Schwann cell)
Negative control
HDAC1/2 cko
Specification
Schwann cell
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Positive control
Control
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Tom Hu

Verified customer

提交于 Mar 21 2012

Western blot abreview for Anti-HDAC2 antibody [3F3]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lymphocyte)
Gel Running Conditions
Reduced Denaturing
Loading amount
500000 cells
Specification
lymphocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 19 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC2 antibody [3F3]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cultured hepatoma cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Cultured hepatoma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 27 2009

ChIP abreview for Anti-HDAC2 antibody [3F3]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (Liver, Hepatoma)
Negative control
Normal mouse IgG
Specification
Liver, Hepatoma
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
Anti RNA polymerase II
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 18 2008

ChIP abreview for Anti-HDAC2 antibody [3F3]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Rat Cell lysate - whole cell (Liver, cultured hepatoma cells)
Negative control
Normal mouse IgG
Specification
Liver, cultured hepatoma cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
Anti RNA polymerase II
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 25 2008

Question

I just wanted to clarify that the vial i was worried about was a brownish colour & not the usual pink.

Regards

Read More
Answer

Thank you for contacting us.

The brownish or pinkish colour is very normal with antibodies if they are not purified. The ab51832 is also in tissue culture supernatant so the solution colour may be different due to presence of culture medium.

The product quality is fully guaranteed so if in case it does not work let us know we will replace the product immediately.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Difference in appearence between the vial.

Read More
Answer

Thank you for contacting us. I apologize for the inconvenience this may have caused.

I have had chat with goods-in department; unfortunately the in-stock vials are no longer available so it is not possible for us to manually check the vials. They however have confirmed that the pinkish appearance is very normal.

I can reassure you that the antibody would be fine. If in any case it fails, please send me an email I will send you a replacement vial the next day.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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