Anti-HCN2抗体[S71] (ab84817)
![Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.cn/ps/products/84/ab84817/Images/ab84817-199537-anti-hcn2-antibody-n71-37-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)")
Key features and details
- Mouse monoclonal [S71] to HCN2
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
概述
-
产品名称
Anti-HCN2抗体[S71]
参阅全部 HCN2 一抗 -
描述
小鼠单克隆抗体[S71] to HCN2 -
宿主
Mouse -
特异性
Does not cross react with HCN1. -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
Fusion protein corresponding to Rat HCN2 aa 750 to the C-terminus (C terminal).
Database link: Q9JKA9 -
阳性对照
- IHC-Fr: Mouse brain tissue; WB: Rat brain membrane and mouse brain tissue lysates;
-
常规说明
The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
S71 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84817于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/1000.
|
|
| ICC/IF | (1) |
1/100.
|
| IHC-P |
1/100.
|
|
| WB | (3) |
1/1000. Predicted molecular weight: 95 kDa.
|
| 说明 |
|---|
|
IHC-Fr
1/1000. |
|
ICC/IF
1/100. |
|
IHC-P
1/100. |
|
WB
1/1000. Predicted molecular weight: 95 kDa. |
靶标
-
功能
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. -
组织特异性
Highly expressed throughout the brain. Detected at low levels in heart. -
序列相似性
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
结构域
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 15166 Mouse
- Entrez Gene: 114244 Rat
- SwissProt: O88703 Mouse
- SwissProt: Q9JKA9 Rat
- Unigene: 12956 Mouse
- Unigene: 224995 Rat
-
别名
- BCNG-2 antibody
- BCNG2 antibody
- Brain cyclic nucleotide gated channel 2 antibody
see all
图片
-
Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)
Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at room temperature.
-
![Western blot - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.cn/ps/products/84/ab84817/Images/ab84817-369636-anti-hcn2-antibody-n71-37-westernblot-mouse-brain.jpg)
Western blot - Anti-HCN2 antibody [S71] (ab84817)Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Mouse brain tissue lysate at 15 µg
Secondary
Goat anti-mouse IgG, HRP at 1/4000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDaChemiluminescent for HRP (Moss) for 5 min in RT.
-
![Western blot - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.cn/ps/products/84/ab84817/Images/ab84817-199538-anti-hcn2-antibody-n71-37-western-blot.jpg)
Western blot - Anti-HCN2 antibody [S71] (ab84817)Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Rat brain membrane lysates at 15 µg
Secondary
HRP-Sheep Anti-Mouse IgG antibody
Predicted band size: 95 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab84817 被引用在 11 文献中.
- Lei X et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
- Luo XD et al. Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel. Neural Regen Res 18:594-602 (2023). PubMed: 36018183
- Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
- Zhang X et al. Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95. Neurosci Res N/A:N/A (2020). PubMed: 32454040
- Zhao H et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175