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Anti-HCN2抗体(ab19346)

Submit a review Q&A (1)References (7)
Western blot - Anti-HCN2 antibody (ab19346)
  • Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (ab19346)

Key features and details

  • Rabbit polyclonal to HCN2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

概述

  • 产品名称

    Anti-HCN2抗体
    参阅全部 HCN2 一抗

  • 描述

    兔多克隆抗体to HCN2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat
    预测可用于: Human

  • 免疫原

    Synthetic peptide corresponding to Human HCN2 aa 874-889.
    Sequence:

    G-LDPQDSARSRLSSNL


    Database link: Q9UL51
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: rat brain lysate ICC/IF: Neuro-2a (Mouse neuroblastoma cell line)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab19346于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/100. Detects a band of approximately 100,115 kDa.
ICC/IF
Use a concentration of 2 µg/ml.
说明
WB
1/100. Detects a band of approximately 100,115 kDa.
ICC/IF
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.

  • 组织特异性

    Highly expressed throughout the brain. Detected at low levels in heart.

  • 序列相似性

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.

  • 结构域

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

图片

  • Western blot - Anti-HCN2 antibody (ab19346)
    Western blot - Anti-HCN2 antibody (ab19346)

    ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

  • Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (ab19346)
    Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (ab19346)

    Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma cell line) cells on 70% confluent log phase labeling HCN2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with ab19346 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

文献 (7)

发表研究结果有使用 ab19346？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab19346 被引用在 7 文献中.

  • Lu Z  et al. Regulation of HCN2 Current by PI3K/Akt Signaling. Front Physiol 11:587040 (2020). PubMed: 33240105
  • Zhao H  et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175
  • Zhang W  et al. Implantation of engineered conduction tissue in the rat heart. Mol Med Rep 19:2687-2697 (2019). PubMed: 30720107
  • Yang L  et al. HCN channel antagonist ZD7288 ameliorates neuropathic pain and associated depression. Brain Res 1717:204-213 (2019). PubMed: 30940440
  • Kretschmannova K  et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . PubMed: 22135067
  • Adams BE  et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol 219:249-57 (2009). WB . PubMed: 19500574
  • Fyk-Kolodziej B & Pourcho RG Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. J Comp Neurol 501:891-903 (2007). PubMed: 17311321

客户评价及客户问答

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Question

Can you tell me where I can purchase the immunising peptide for this antibody and what their Western blot protocol is including the preparation of lysates?

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Answer

Thank you for your enquiry. Unfortunately we do not sell the immunising peptide for this antibody. The Western blot protocol used to test this antibody can be found under our Protocol tab. Regarding lysate prep, you should be able to use a standard lysis buffer (75 mM Tris-HCl, pH 7.2, 200 mM NaCl, 1% Tween 100, 100 mM EDTA, 1 mM DTT with protease inhibitors). Please note, HCN2 has an N-linked glycosylation in the brain. This antibody detects a band at ~115 kDa (due to glycosylation). However this band can be reduced to ~100 kDa by the addition of PNGase F. You may find the deglycosylation procedure in this specific reference. http://www.jbc.org/cgi/reprint/M306958200v1 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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