Anti-Hck (phospho Y410)抗体(ab61055)
Key features and details
- Rabbit polyclonal to Hck (phospho Y410)
- Suitable for: ELISA, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Hck (phospho Y410)抗体
参阅全部 Hck 一抗 -
描述
兔多克隆抗体to Hck (phospho Y410) -
宿主
Rabbit -
特异性
Detects target only when phosphorylated at tyrosine 410. -
经测试应用
适用于: ELISA, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide within Human Hck aa 350-450 (phospho Y410). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P08631 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61055于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils. -
组织特异性
Expressed predominantly in cells of the myeloid and B-lymphoid lineages. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates binding to HIV-1 Nef. -
细胞定位
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3. - Information by UniProt
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数据库链接
- Entrez Gene: 3055 Human
- Entrez Gene: 15162 Mouse
- Entrez Gene: 25734 Rat
- Omim: 142370 Human
- SwissProt: P08631 Human
- SwissProt: P08103 Mouse
- SwissProt: P50545 Rat
- Unigene: 655210 Human
see all -
形式
This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- Bmk antibody
- Hck 1 antibody
- Hck antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Hck (phospho Y410) antibody (ab61055)
1/500-1/1000 ab61055 staining phosphorylated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab61055 被引用在 5 文献中.
- Kim H et al. IgSF11-mediated phosphorylation of pyruvate kinase M2 regulates osteoclast differentiation and prevents pathological bone loss. Bone Res 11:17 (2023). PubMed: 36928396
- Chen M et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. Nat Commun 14:4297 (2023). PubMed: 37463911
- Roversi FM et al. Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis. Front Cell Dev Biol 9:634044 (2021). PubMed: 33842460
- Kao TI et al. Bletinib ameliorates neutrophilic inflammation and lung injury by inhibiting Src family kinase phosphorylation and activity. Br J Pharmacol 178:4069-4084 (2021). PubMed: 34131920
- Poh AR et al. Inhibition of Hematopoietic Cell Kinase Activity Suppresses Myeloid Cell-Mediated Colon Cancer Progression. Cancer Cell 31:563-575.e5 (2017). PubMed: 28399411