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重组RabMAb

重组Anti-Hck抗体[EP2415Y] (ab75839)

Submit a review Q&A (1)References (3)
Western blot - Anti-Hck antibody [EP2415Y] (ab75839)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EP2415Y] to Hck
    • Suitable for: WB
    • Reacts with: Human

    Conjugates logo Related conjugates and formulations

    Carrier Free

    概述

    • 产品名称

      Anti-Hck抗体[EP2415Y]
      参阅全部 Hck 一抗

    • 描述

      兔单克隆抗体[EP2415Y] to Hck

    • 宿主

      Rabbit

    • 特异性

      This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • WB: TF-1 and U-937 whole cell lysates.

    • 常规说明

      This product has switched from a hybridoma to recombinant production method on 13th September 2022.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab75839于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/20000. Predicted molecular weight: 59 kDa.
    说明
    WB
    1/20000. Predicted molecular weight: 59 kDa.

    靶标

    • 功能

      May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.

    • 组织特异性

      Expressed predominantly in cells of the myeloid and B-lymphoid lineages.

    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • 结构域

      The SH3 domain mediates binding to HIV-1 Nef.

    • 细胞定位

      Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
    • Information by UniProt

    • 数据库链接

      • 形式

        This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

      • 别名

        • Bmk antibody
        • Hck 1 antibody
        • Hck antibody
        • HCK_HUMAN antibody
        • Hemopoietic cell kinase antibody
        • JTK9 antibody
        • p59-HCK/p60-HCK antibody
        • p59Hck antibody
        • p59HCK/p60HCK antibody
        • p61Hck antibody
        • Tyrosine protein kinase HCK antibody
        • Tyrosine-protein kinase HCK antibody
        see all

      图片

      • Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        All lanes : Anti-Hck antibody [EP2415Y] (ab75839) at 1/20000 dilution

        Lane 1 : TF-1 (Human bone marrow erythroblast) whole cell lysate
        Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 59 kDa
        Observed band size: 59 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second (Lane 1); 3 seconds (Lane 2).

      文献 (3)

      发表研究结果有使用 ab75839？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab75839 被引用在 3 文献中.

      • Sakoda T  et al. TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia. Blood Adv 7:2053-2065 (2023). PubMed: 36745103
      • Chen M  et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. Nat Commun 14:4297 (2023). PubMed: 37463911
      • Roversi FM  et al. Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis. Front Cell Dev Biol 9:634044 (2021). PubMed: 33842460

      客户评价及客户问答

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      Question

      This antibody (cat #2254-1) looks highly interesting. However, as the C-terminal region of the protein shares homology with other SRC-related tyrosine kinases, I wonder whether specificity against HCK vs SRC, LCK, YES, LYN, FYN, FGR and BLK has been tested. Can you provide me this information?  

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      Answer

      We have not tested ab75839 in-house for cross reactivity with LCK,YES, or BLK so we cannot say for certain whether this antibody would be suitable for detecting these targets. However, in our WB tests we have only observed a single band at ~ 60 KDa when testing the antibody within our recommended dilution range of 1:20,000-200,000. There was one instance when we tested a much higher concentration (1:1,000) and observed an additional band at ~ 140 KDa but it was likely non-specific.

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