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重组RabMAb

重组Anti-hCG beta抗体[EPR28726-38] - BSA and Azide free (ab317568)

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Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
  • Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
  • Indirect ELISA - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
  • Dot Blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
  • Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28726-38] to hCG beta - BSA and Azide free
  • Suitable for: WB, Dot blot, Indirect ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-hCG beta抗体[EPR28726-38] - BSA and Azide free
    参阅全部 hCG beta 一抗

  • 描述

    兔单克隆抗体[EPR28726-38] to hCG beta - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This antibody could recognize human CGB3, CGB2, CGB7 and CGB1.

  • 经测试应用

    适用于: WB, Dot blot, Indirect ELISAmore details
    不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human placenta lysates. Dot blot: 293T cells transfected with a human CGB3 expression vector containing a His-tag, 293T cells transfected with a human CGB2 expression vector containing a His-tag, 293T cells transfected with a human CGB7 expression vector containing a His-tag, 293T cells transfected with a human CGB1 expression vector containing a His-tag whole cell lysate.

  • 常规说明

    ab317568 is the carrier-free version of ab317567.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317568于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

靶标

  • 相关性

    Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Tissue specificity: Placenta. Similarity: Belongs to the glycoprotein hormones subunit beta family. Developmental stage: Made by the first trimester placenta.

  • 数据库链接

    • 别名

      • CG beta antibody
      • CG-beta antibody
      • CgB antibody
      • CGB3 antibody
      • CGB3_HUMAN antibody
      • CGB5 antibody
      • CGB7 antibody
      • CGB7_HUMAN antibody
      • CGB8 antibody
      • CGHB_HUMAN antibody
      • Choriogonadotropin subunit beta 3 antibody
      • Choriogonadotropin subunit beta 7 antibody
      • Choriogonadotropin subunit beta antibody
      • Chorionic gonadotropin beta polypeptide antibody
      • Chorionic gonadotropin chain beta antibody
      see all

    图片

    • Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      All lanes : Anti-hCG beta antibody [EPR28726-38] (ab317567) at 1/1000 dilution

      Lane 1 : Untreated JEG-3 (human placenta epithelial cell) whole cell lysate
      Lane 2 : JEG-3 treated with 100uM Forskolin for 24h whole cell lysate
      Lane 3 : Untreated JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate
      Lane 4 : JAR treated with 100uM Forskolin for 24h whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Observed band size: 19,21 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This data was developed using ab317567, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer: 5% NFDM/TBST.

      ab181602 was used as a loading control at 1/200000 dilution.

      This antibody could detect 2 isoforms produced by alternative splicing.

      The expression of hCG beta is upregulated in response to Forskolin treatment (PMID: 23598405)

    • Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      Western blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      All lanes : Anti-hCG beta antibody [EPR28726-38] (ab317567) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human liver tissue lysate
      Lane 3 : Human heart tissue lysate
      Lane 4 : Human kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Observed band size: 19,21 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab317567, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: liver (PMID: 24309898), heart (PMID: 24309898), kidney (PMID: 24309898).

      This antibody could detect 2 isoforms produced by alternative splicing.

      The identity of the higher MW band at approximately 30 kDa is unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    • Indirect ELISA - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      Indirect ELISA - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)

      This data was developed using ab317567, the same antibody clone in a different buffer formulation.

      Indirect ELISA analysis of ab317567 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

      Antigen: hCG beta.

      Antigen concentration: 1000 ng/ml

    • Dot Blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      Dot Blot - Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)

      This data was developed using ab317567, the same antibody clone in a different buffer formulation.

      Dot blot analysis of hCG beta using ab317567 at 1:1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:20,000 dilution.

      Lane 1: 293T cells transfected with a human CGB3 expression vector containing a His-tag whole cell lysate

      Lane 2: 293T cells transfected with a human CGB2 expression vector containing a His-tag whole cell lysate

      Lane 3: 293T cells transfected with a human CGB7 expression vector containing a His-tag whole cell lysate

      Lane 4: 293T cells transfected with a human CGB1 expression vector containing a His-tag whole cell lysate

      Exposure time: 10 seconds.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody could recognize human CGB3, CGB2, CGB7 and CGB1.

    • Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)
      Anti-hCG beta antibody [EPR28726-38] - BSA and Azide free (ab317568)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317568？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317568 尚未被引用在任何文献中。

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