AB201046

重组Anti-HCE抗体[EPR19384]

Name: HCE抗体[EPR19384] (ab201046)| Abcam中文官网
Brand: Abcam Limited
SKU: AB201046
Price: 2279 CNY
Availability: InStock
Rating: 5 (5 reviews)

Anti-HCE antibody [EPR19384]

RabMAb
Recombinant
了解详情

(5 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal HCE antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

查看别名

CAP1A, RNGTT, mRNA-capping enzyme, HCAP1, HCE

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-HCE antibody [EPR19384] (AB201046)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCE antibody [EPR19384] (AB201046)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling HCE with ab201046 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR19384] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab201046 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-HCE antibody [EPR19384] (AB201046)

HCE was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab201046 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab201046 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : SH-SY5Y whole cell lysate 10μg (Input).
Lane 2 : ab201046 IP in SH-SY5Y whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR19384] -Isotype Control (ab172730) instead of ab201046 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-HCE antibody [EPR19384] (ab201046)

Predicted band size: 69 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCE antibody [EPR19384] (AB201046)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCE antibody [EPR19384] (AB201046)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HCE with ab201046 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCE antibody [EPR19384] (AB201046)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling HCE with ab201046 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

The negative controls are as follows :
-ve control 1 : ab201046 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCE antibody [EPR19384] (AB201046)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling HCE with ab201046 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCE antibody [EPR19384] (ab201046) at 1/2000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCE antibody [EPR19384] (ab201046) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCE antibody [EPR19384] (ab201046) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCE antibody [EPR19384] (ab201046) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 30s

不同偶联物与剂型 (1)

Carrier free

Anti-HCE antibody [EPR19384] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19384

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "The IHC application is recommended for mouse and rat only." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "The IHC application is recommended for mouse and rat only." } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HCE also known as Heat Shock Cognate 70 is a stress-responsive protein with a mass of approximately 70 kDa. This protein plays a mechanical role as a molecular chaperone assisting in the proper folding of nascent polypeptides and the refolding of misfolded proteins. HCE expression occurs in various tissues but notably it is abundant in the cytoplasm of cells exposed to stressful conditions like heat shock. The protein assists in maintaining cellular protein homeostasis particularly during periods of increased environmental stress.

Biological function summary

HCE contributes to multiple cellular processes critical for survival and adaptation. It is an important component of the chaperone complex and interacts with co-chaperones to facilitate protein folding and prevent aggregation. HCE enhances cellular resilience by stabilizing proteins and protecting against cellular stress-induced damage. Additionally it supports proteostasis by targeting misfolded proteins for degradation via the ubiquitin-proteasome system.

Pathways

HCE is involved in essential cellular mechanisms beyond protein folding including the unfolded protein response (UPR) and heat shock response (HSR). In these pathways HCE collaborates with proteins like HSP40 and HSP90 to restore normal protein conformation and function. The heat shock response pathway is a protective cellular mechanism that activates a cascade to manage stress-related protein damage and ensure cell survival during acute stress.

HCE has significant connections to neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Research indicates that aberrant HCE function may contribute to protein aggregation a hallmark of these conditions. In Alzheimer’s disease HCE interacts with proteins like tau and amyloid precursor protein (APP) influencing pathological aggregation processes. Understanding the role of HCE in these disorders opens potential avenues for therapeutic targeting to mitigate protein aggregation and fibrous plaques characteristic of neurodegeneration.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation : by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.

See full target information RNGTT

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 135: PubMed34897463

2022

Comparative analysis of vertebrates reveals that mouse primordial oocytes do not contain a Balbiani body.

Applications

Unspecified application

Species

Unspecified reactive species

Laasya Dhandapani,Marion C Salzer,Juan M Duran,Gabriele Zaffagnini,Cristian De Guirior,Maria Angeles Martínez-Zamora,Elvan Böke

EMBO molecular medicine 13:e13158 PubMed33369168

2020

Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Leandro R Soria,Sonam Gurung,Giulia De Sabbata,Dany P Perocheau,Angela De Angelis,Gemma Bruno,Elena Polishchuk,Debora Paris,Paola Cuomo,Andrea Motta,Michael Orford,Youssef Khalil,Simon Eaton,Philippa B Mills,Simon N Waddington,Carmine Settembre,Andrés F Muro,Julien Baruteau,Nicola Brunetti-Pierri

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-HCE抗体[EPR19384]

Anti-HCE antibody [EPR19384]

Immunocytochemistry/ Immunofluorescence - Anti-HCE antibody [EPR19384] (AB201046)

Immunoprecipitation - Anti-HCE antibody [EPR19384] (AB201046)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCE antibody [EPR19384] (AB201046)

Immunocytochemistry/ Immunofluorescence - Anti-HCE antibody [EPR19384] (AB201046)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCE antibody [EPR19384] (AB201046)

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Western blot - Anti-HCE antibody [EPR19384] (AB201046)

Western blot - Anti-HCE antibody [EPR19384] (AB201046)