重组Anti-HAUSP / USP7抗体[EPR4253]
Anti-HAUSP / USP7 antibody [EPR4253]
- RabMAb
- Recombinant
- KO Validated
- 了解详情
4
(2 Reviews)
|
(21 Publications)
Rabbit Recombinant Monoclonal HAUSP / USP7 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 21 publications.
查看别名
HAUSP, USP7, Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
ab108931, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HAUSP / USP7 with unpurified ab108931 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
ab108931, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
Lanes 1- 2 : Merged signal (red and green). Green - ab108931 observed at 128 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108931 was shown to react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266535 (knockout cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and USP7 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108931 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
USP7 knockout HEK293T cell lysate at 20 µg
Predicted band size: 128 kDa
Observed band size: 128 kDa
false
- WB
Unknown
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
All lanes:
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1:
Ramos cell lysate at 10 µg
Lane 2:
HeLa cell lysate at 10 µg
Lane 3:
A431 cell lysate at 10 µg
Lane 4:
MCF7 cell lysate at 10 µg
Lane 5:
C6 cell lysate at 10 µg
Lane 6:
RAW 264.7 cell lysate at 10 µg
Predicted band size: 128 kDa
false
- WB
Lab
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : HAUSP/USP7 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108931 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108931 was shown to specifically react with HAUSP/USP7 when HAUSP/USP7 knockout samples were used. Wild-type and HAUSP/USP7 knockout samples were subjected to SDS-PAGE. ab108931 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)
Predicted band size: 128 kDa
false
- WB
Lab
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
Lanes 1- 2 : Merged signal (red and green). Green - ab108931 observed at 128 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108931 was shown to react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266535 (CRISPR/Cas9 edited cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and USP7 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108931 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
USP7 CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human USP7 (HAUSP) knockout HEK-293T cell line (<a href='/products/cell-lines/human-usp7-hausp-knockout-hek-293t-cell-line-ab266535'>ab266535</a>)
Predicted band size: 128 kDa
Observed band size: 128 kDa
false
- WB
Lab
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) 1 : 1000,000 dilution.
Exposure time : Lane 1 : 60 seconds; Lane 2 : 3 seconds
All lanes:
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1:
Rat spinal cord tissue lysate at 20 µg
Lane 2:
PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 128 kDa
false
不同偶联物与剂型 (10)
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-HAUSP / USP7 antibody [EPR4253]
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Anti-HAUSP / USP7 antibody [EPR4253] - BSA and Azide free
-
578 PE
PE Anti-HAUSP / USP7 antibody [EPR4253]
-
660 APC
APC Anti-HAUSP / USP7 antibody [EPR4253]
-
HRP Anti-HAUSP / USP7 antibody [EPR4253]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-HAUSP / USP7 antibody [EPR4253]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-HAUSP / USP7 antibody [EPR4253]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-HAUSP / USP7 antibody [EPR4253]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-HAUSP / USP7 antibody [EPR4253]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-HAUSP / USP7 antibody [EPR4253]
反应性数据
产品详情
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HAUSP influences several critical cellular processes including DNA repair transcriptional regulation and cell cycle progression. It often interacts with other proteins such as p53 to modify their functions by altering their ubiquitination status. HAUSP does not function in isolation but forms part of larger protein complexes where it plays a role in processing substrate proteins. Its ability to activate or deactivate proteins through deubiquitination highlights its importance in maintaining cellular homeostasis.
Pathways
Research identifies HAUSP as an important component in both the p53 pathway and the Wnt signaling pathway. Through the p53 pathway HAUSP directly interacts with the p53 tumor suppressor protein influencing cell cycle and apoptosis decisions. Its participation in the Wnt signaling pathway associates it with beta-catenin where it supports cellular proliferation and differentiation. These pathway interactions illustrate HAUSP's integral role in maintaining cellular function and responding to various signaling inputs.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (21)
Recent publications for all applications. Explore the full list and refine your search
Theranostics 15:9819-9837 PubMed41041071
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Hereditas 162:44 PubMed40121492
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Nature communications 16:2526 PubMed40087304
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Open life sciences 19:20220929 PubMed39310812
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Journal of applied toxicology : JAT 44:1949-1961 PubMed39142713
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iScience 27:109693 PubMed38689642
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Biomedicines 12: PubMed38672118
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