重组Anti-hair cortex Cytokeratin/K40抗体[AE13]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat skin. The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on rat cerebrum.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse skin (PMID : 2432071). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Blocking and diluting buffer : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat skin tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false