Anti-HADHB抗体[5C3AB7] (ab110301)
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.cn/ps/products/110/ab110301/Images/ab110301-242584-anti-hadhb-antibody-5c3ab7-western-blot.jpg "Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)")
Key features and details
- Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: IP, WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-HADHB抗体[5C3AB7]
参阅全部 HADHB 一抗 -
描述
小鼠单克隆抗体[5C3AB7] to HADHB -
宿主
Mouse -
经测试应用
适用于: IP, WB, Flow Cyt, ICCmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2 and HLM cell lysates; HDFn and HeLa cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
克隆
单克隆 -
克隆编号
5C3AB7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110301于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC
Use a concentration of 1 µg/ml. |
靶标
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通路
Lipid metabolism; fatty acid beta-oxidation. -
疾病相关
Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex. -
序列相似性
Belongs to the thiolase family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 3032 Human
- Omim: 143450 Human
- SwissProt: P55084 Human
- Unigene: 515848 Human
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别名
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
see all
图片
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Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa -
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.cn/ps/products/110/ab110301/Images/ab110301-123552-anti-hadhb-antibody-5c3ab7.gif)
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate
Predicted band size: 51 kDaAs a Western blot antibody, ab110301 works with HepG2 (human) cells.
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![Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.cn/ps/products/110/ab110301/Images/ab110301-123551-anti-hadhb-antibody-5c3ab7-immunocytochemistry.jpg)
Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue). -
![Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.cn/ps/products/110/ab110301/Images/ab110301-123550-anti-hadhb-antibody-5c3ab7-flow-cytometry.gif)
Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red). -
![Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.cn/ps/products/110/ab110301/Images/ab110301-123549-anti-hadhb-antibody-5c3ab7-immunoprecipitation.gif)
Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)As an IP antibody, MS733 works with human samples.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab110301 被引用在 2 文献中.
- Lu J et al. Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma. Front Oncol 11:737127 (2021). PubMed: 34976793
- Li XX et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065