Anti-HADH抗体[1A12BC8] (ab110284)
Key features and details
- Mouse monoclonal [1A12BC8] to HADH
- Suitable for: IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-HADH抗体[1A12BC8]
参阅全部 HADH 一抗 -
描述
小鼠单克隆抗体[1A12BC8] to HADH -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IF, IP, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HL-60 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purity near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
1A12BC8 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8. |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. -
组织特异性
Expressed in liver, kidney, pancreas, heart and skeletal muscle. -
通路
Lipid metabolism; fatty acid beta-oxidation. -
疾病相关
Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency) [MIM:231530]. HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death.
Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4) [MIM:609975]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion. -
序列相似性
Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3033 Human
- Omim: 601609 Human
- SwissProt: Q16836 Human
- Unigene: 438289 Human
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别名
- 3 hydroxyacyl Coenzyme A dehydrogenase antibody
- HAD antibody
- HADH antibody
see all
图片
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MRC5 fibroblasts labeled with ab110284 at 5µg/ml and stained with Texas Red conjugated to goat anti-mouse secondary.
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HADH immunocaptured from Human liver mitochondria using ab110284.
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HL-60 cells were stained with 1 µg/ml ab110284 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody [1A12BC8] (ab110284)
ab110284, at 1/250 dilution, staining HADH in formalin-fixed, paraffin-embedded Human cerebellum by Immunohistochemistry. HADH immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab110284 被引用在 1 文献中.
- Zhang Q et al. Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma. Front Immunol 14:1324205 (2023). PubMed: 38090559