重组Anti-HACE1抗体[EPR7962] (ab133637)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7962] to HACE1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HACE1抗体[EPR7962]
参阅全部 HACE1 一抗 -
描述
兔单克隆抗体[EPR7962] to HACE1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
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阳性对照
- 293T and SH-SY5Y cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7962 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133637于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
靶标
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相关性
E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor. -
细胞定位
Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. A significant portion localizes to the endoplasmic reticulum. -
数据库链接
- Entrez Gene: 57531 Human
- Entrez Gene: 209462 Mouse
- Entrez Gene: 361866 Rat
- Omim: 610876 Human
- SwissProt: Q8IYU2 Human
- SwissProt: Q3U0D9 Mouse
- SwissProt: D3ZBM7 Rat
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别名
- E3 ubiquitin-protein ligase HACE1 antibody
- HACE 1 antibody
- HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 antibody
- KIAA1320 antibody
图片
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Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab133637 被引用在 9 文献中.
- Jiang HY et al. Ubiquitylation of cyclin C by HACE1 regulates cisplatin-associated sensitivity in gastric cancer. Clin Transl Med 12:e770 (2022). PubMed: 35343092
- Palicharla VR et al. Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion. FEBS Lett 595:491-506 (2021). PubMed: 33421097
- Li S et al. Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells. Oncol Lett 20:280 (2020). PubMed: 33014158
- Kogler M et al. HACE1 Prevents Lung Carcinogenesis via Inhibition of RAC-Family GTPases. Cancer Res 80:3009-3022 (2020). PubMed: 32366477
- Goka ET et al. Rac Signaling Drives Clear Cell Renal Carcinoma Tumor Growth by Priming the Tumor Microenvironment for an Angiogenic Switch. Mol Cancer Ther 19:1462-1473 (2020). PubMed: 32371578
- El-Naggar AM et al. HACE1 is a potential tumor suppressor in osteosarcoma. Cell Death Dis 10:21 (2019). PubMed: 30622235
- Yu Z et al. Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncol Lett 17:4361-4368 (2019). PubMed: 30988809
- Goka ET & Lippman ME Loss of the E3 ubiquitin ligase HACE1 results in enhanced Rac1 signaling contributing to breast cancer progression. Oncogene 34:5395-405 (2015). WB ; Human . PubMed: 25659579
- Hollstein R et al. HACE1 deficiency causes an autosomal recessive neurodevelopmental syndrome. J Med Genet 52:797-803 (2015). WB . PubMed: 26424145