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AB308398

重组Anti-HA tag抗体[HL1603]

Anti-HA tag antibody [HL1603]

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Rabbit Recombinant Monoclonal HA tag antibody. Carrier free. Suitable for I-ELISA, IHC-P, ICC/IF, WB and reacts with Tag samples.

查看别名

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (AB308398)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (AB308398)

Immunohistochemical analysis of mock and HA tag - transfected HEK293T cell pellet labeling HA tag using ab308398 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (AB308398)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (AB308398)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HA tag using ab308398 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Indirect ELISA - Anti-HA tag antibody [HL1603] (AB308398)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-HA tag antibody [HL1603] (AB308398)

Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (ab308398) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1/10000) was used to detect the bound primary antibody.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1603

亚型

IgG

不含载体蛋白

Yes

应用

I-ELISA, IHC-P, WB, ICC/IF

applications

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搭配使用产品

Primary Antibodies

AB13834

Anti-HA tag antibody

Lab Essentials

Western blot - Anti-HA tag antibody (AB13834)

  • 5
  • 3
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB9106

Anti-Myc tag antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

  • 4
  • 39
Primary Antibodies

AB9110

Anti-HA tag antibody - ChIP Grade

Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

  • 5
  • 59
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information HA tag
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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