Anti-HA tag抗体[HA.C5] (ab18181)
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-212146-anti-ha-tag-antibody-hac5-western-blot.jpg "Western blot - Anti-HA tag antibody [HA.C5] (ab18181)")
Key features and details
- Mouse monoclonal [HA.C5] to HA tag
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG3
概述
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产品名称
Anti-HA tag抗体[HA.C5]
参阅全部 HA tag 一抗 -
描述
小鼠单克隆抗体[HA.C5] to HA tag -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide from influenza hemagglutinin epitope:
YPYDVPDYA
conjugated to KLH. -
常规说明
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
HA.C5 -
同种型
IgG3 -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18181于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (12) |
1/1000.
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/1000. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1-2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26826-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chaharab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26829-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26828-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26827-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (269)
ab18181 被引用在 269 文献中.
- Wang X et al. STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells. Commun Biol 7:10 (2024). PubMed: 38172644
- Sun Y et al. A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins. Microb Cell Fact 22:194 (2023). PubMed: 37749574
- Jiang R et al. TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates. iScience 26:108315 (2023). PubMed: 38025781
- Wallace I et al. Insights into the ISG15 transfer cascade by the UBE1L activating enzyme. Nat Commun 14:7970 (2023). PubMed: 38042859
- Bailey-Elkin BA et al. Demonstrating the importance of porcine reproductive and respiratory syndrome virus papain-like protease 2 deubiquitinating activity in viral replication by structure-guided mutagenesis. PLoS Pathog 19:e1011872 (2023). PubMed: 38096325