AB18181

Anti-HA tag 抗体 [HA.C5]

Name: HA tag抗体[HA.C5] (ab18181)| Abcam中文官网
Brand: Abcam Limited
SKU: AB18181
Rating: 4 (19 reviews)

Anti-HA tag antibody [HA.C5]

Lab Essentials
了解详情

(19 Reviews)

(269 Publications)

Anti-HA tag antibody [HA.C5] (ab18181) is a mouse monoclonal antibody detecting HA tag in Western Blot, ICC/IF.

- Clone HA.C5 is the most cited clone to HA tag
- Over 260 publications
- Trusted since 2005

查看别名

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

5 Images

AbReview39578****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

Lanes 1 - 2:

HEK293 whole cell lysate - transfected at 30 µg

Lane 3:

HEK293 whole cell lysate - untransfected at 30 µg

Secondary

All lanes:

IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 85 kDa

false

Exposure time: 5min

This image is courtesy of an anonymous Abreview

AbReview5311****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

Lane 1:

WCE from cell line transfected for HA-tagged protein at 50 µg

Lane 2:

WCE from a cell line transfected with empty vector at 50 µg

Secondary

All lanes:

HRP conjugated Goat anti-mouse IgG (H+L)

Predicted band size: 64 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).

ICC/IF

AbReview31652****

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

This image is courtesy of an Abreview submitted by Harendra Chahar

Unknown

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

Predicted band size: 64 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HA.C5

亚型

IgG3

不含载体蛋白

应用

WB, ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from TCS

存储溶液

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information HA tag

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文献 (269)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:10 PubMed38172644

2024

STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Wang,Chao Han,Di Yang,Jian Zhou,Hui Dong,Zhiyuan Wei,Shuai Xu,Chen Xu,Yiwei Zhang,Yi Sun,Bing Ni,Sheng Guo,Jingbo Zhang,Tingting Zhao,Xiangmei Chen,Jie Luo,Yuzhang Wu,Yi Tian

Scientific reports 13:22952 PubMed38135735

2023

TRIM25 dictates selective miRNA loading into extracellular vesicles during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla E King,Priyanka Ghosh,Ann L Wozniak

PLoS pathogens 19:e1011872 PubMed38096325

2023

Demonstrating the importance of porcine reproductive and respiratory syndrome virus papain-like protease 2 deubiquitinating activity in viral replication by structure-guided mutagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ben A Bailey-Elkin,Robert C M Knaap,Anuradha De Silva,Ilse M Boekhoud,Sandra Mous,Niek van Vught,Mazdak Khajehpour,Erwin van den Born,Marjolein Kikkert,Brian L Mark

Nature communications 14:7970 PubMed38042859

2023

Insights into the ISG15 transfer cascade by the UBE1L activating enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Iona Wallace,Kheewoong Baek,J Rajan Prabu,Ronnald Vollrath,Susanne von Gronau,Brenda A Schulman,Kirby N Swatek

iScience 26:108315 PubMed38025781

2023

TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Jiang,Wentao Zhu,Zhiwei Liao,Chunrong Yang,Jianguo Su

Microbial cell factories 22:194 PubMed37749574

2023

A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Sun,Yongrong Zhang,Hua Yu,Ashley Saint Fleur,Di Yu,Zhiyong Yang,Hanping Feng

mBio 14:e0061123 PubMed37712680

2023

Interferon-stimulated gene TDRD7 interacts with AMPK and inhibits its activation to suppress viral replication and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sukanya Chakravarty,Gayatri Subramanian,Sonam Popli,Manoj Veleeparambil,Shumin Fan,Ritu Chakravarti,Saurabh Chattopadhyay

Nature communications 14:3126 PubMed37253751

2023

Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family.

Applications

Unspecified application

Species

Unspecified reactive species

Fanindra Kumar Deshmukh,Gili Ben-Nissan,Maya A Olshina,Maria G Füzesi-Levi,Caley Polkinghorn,Galina Arkind,Yegor Leushkin,Irit Fainer,Sarel J Fleishman,Dan Tawfik,Michal Sharon

Journal of clinical immunology 43:1403-1413 PubMed37156989

2023

Insights into the expanding intestinal phenotypic spectrum of SOCS1 haploinsufficiency and therapeutic options.

Applications

Unspecified application

Species

Unspecified reactive species

Marco M Rodari,Dominique Cazals-Hatem,Mathieu Uzzan,Nicolas Martin Silva,Anis Khiat,Minh Chau Ta,Ludovic Lhermitte,Aurore Touzart,Sylvain Hanein,Cléa Rouillon,Francisca Joly,Adrienne Elmorjani,Julie Steffann,Nadine Cerf-Bensussan,Marianna Parlato,Fabienne Charbit-Henrion

Nature communications 14:1768 PubMed36997511

2023

The plant unique ESCRT component FREE1 regulates autophagosome closure.

Applications

Unspecified application

Species

Unspecified reactive species

Yonglun Zeng,Baiying Li,Shuxian Huang,Hongbo Li,Wenhan Cao,Yixuan Chen,Guoyong Liu,Zhenping Li,Chao Yang,Lei Feng,Jiayang Gao,Sze Wan Lo,Jierui Zhao,Jinbo Shen,Yan Guo,Caiji Gao,Yasin Dagdas,Liwen Jiang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HA tag 抗体 [HA.C5]

Anti-HA tag antibody [HA.C5]

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

应用

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

产品实验方案

靶点信息

文献 (269)

Abcam Product Promise

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