AB182009

重组Anti-HA tag抗体[EPR4095]

Anti-HA tag antibody [EPR4095]

RabMAb
Recombinant
Lab Essentials
了解详情

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal HA tag antibody. Suitable for WB and reacts with Tag samples. Cited in 6 publications.

查看别名

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

2 Images

Supplier Data

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HA tag antibody [EPR4095] (ab182009) at 1/5000 dilution

All lanes:

multi-tag fusion protein, 1 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

false

Lab

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

All lanes:

Western blot - Anti-HA tag antibody [EPR4095] (ab182009) at 1/10000 dilution

All lanes:

HA-tagged Human Glutaminase C overexpression lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 57 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-HA tag antibody [EPR4095] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4095

亚型

IgG

不含载体蛋白

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information HA tag

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 51:102181 PubMed39541710

2024

Methionine restriction promotes the polarization of macrophages towards M1 and the immunotherapy effect of PD-L1/PD-1 blockades by inhibiting the secretion of MIF by gastric carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xin,Jiang Liu,Jun-Yan Lai,He-Song Xu,Luo-Jun Fan,Yong-Hui Zou,Qi Zhou,Zhen-Qi Yue,Jin-Heng Gan

Frontiers in cell and developmental biology 10:833396 PubMed35669517

2022

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Bo Jiang,Kangli Peng,Shasha Li,Xiangnan Liu,Bufan Wang,Yuntian Chen,Tiepeng Wang,Bo Zhao

Cell cycle (Georgetown, Tex.) 20:1692-1707 PubMed34334113

2021

ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbao Guo,Manli Xie,Miao Guo,Feiping Yan,Lihong Li,Rui Liu

EMBO reports 21:e51869 PubMed33274589

2020

Atg1 kinase regulates autophagosome-vacuole fusion by controlling SNARE bundling.

Applications

Unspecified application

Species

Unspecified reactive species

Saskia Barz,Franziska Kriegenburg,Anna Henning,Anuradha Bhattacharya,Hector Mancilla,Pablo Sánchez-Martín,Claudine Kraft

Journal of cell science 132: PubMed31649143

2019

Vac8 spatially confines autophagosome formation at the vacuole in .

Applications

Unspecified application

Species

Unspecified reactive species

David M Hollenstein,Rubén Gómez-Sánchez,Akif Ciftci,Franziska Kriegenburg,Muriel Mari,Raffaela Torggler,Mariya Licheva,Fulvio Reggiori,Claudine Kraft

The Journal of biological chemistry 290:3529-41 PubMed25519908

2014

Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin.

Applications

Unspecified application

Species

Human

Kathryn H Driesbaugh,Marguerite S Buzza,Erik W Martin,Gregory D Conway,Joseph P Y Kao,Toni M Antalis

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-HA tag抗体[EPR4095]