Anti-HA tag抗体(ab9111)
Key features and details
- Chicken polyclonal to HA tag
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: unknown
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HA tag抗体
参阅全部 HA tag 一抗 -
描述
鸡多克隆抗体to HA tag -
宿主
Chicken -
特异性
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate. -
经测试应用
适用于: WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA (epitope)
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阳性对照
- ICC/IF: CHO cells transfected with 12 tags constructs (CHO-12 tags) and transfected with GFP-HA constructs (CHO-GFP-HA).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 4 - 5 µg/ml.
|
| 说明 |
|---|
|
WB
1/1000. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 4 - 5 µg/ml. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilised with 0.1% Triton X-100 in cells transfected with GFP-HA contrsucts (as CHO-GFP-HA) or parental non- transfected CHO cells labelling with chicken anti-HA tag (ab9111) at 5µg/ml, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5µg/ml (Red). Nucleus was counterstained with DAPI (Blue).
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling HA tag with ab9111 at 1/250 dilution (4μg/mL), followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 1/500 dilution (2 μg/mL) (Red). Nucleus was counterstained with DAPI (white). Bottom panel images showed Parallel staining in parental CHO cell line.
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9111 at 5μg/mL, followed by Goat Anti- Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 μg/mL (Red). Nucleus was counterstained with DAPI (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab9111 被引用在 21 文献中.
- Zeballos C MA et al. Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins. Nat Commun 14:6492 (2023). PubMed: 37838698
- Zhao B et al. Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration. Acta Neuropathol Commun 11:200 (2023). PubMed: 38111057
- Wendt L et al. Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1. J Virol 96:e0090022 (2022). PubMed: 36040180
- Powell JE et al. Targeted gene silencing in the nervous system with CRISPR-Cas13. Sci Adv 8:eabk2485 (2022). PubMed: 35044815
- Decombe S et al. Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability. Epigenetics Chromatin 14:35 (2021). PubMed: 34321103