Anti-HA tag抗体(ab137838)
Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: WB, ICC/IF, IP
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HA tag抗体
参阅全部 HA tag 一抗 -
描述
兔多克隆抗体to HA tag -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Species independent -
免疫原
A synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA.
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阳性对照
- HA-recombinant protein transfected HeLa cells; HA-tagged protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137838于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Western blot - Anti-HA tag antibody (ab137838)All lanes : Anti-HA tag antibody (ab137838) at 1/2000 dilution
Lane 1 : Cell extracts from non-transfected cell line
Lane 2 : Cell extracts from cell line transfected for HA-tagged protein
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab137838)Immunofluorescence analysis of transfected paraformaldehyde-fixed HeLa cells, labelling HA-recombinant protein using ab137838 antibody at 1/500 dilution. Right panel costained with Hoechst 33342. -
Immunoprecipitation - Anti-HA tag antibody (ab137838)HA-tagged protein was immunoprecipitated from transfected cells using 5 ug of ab137838. The precipitated HA-tagged protein was detected using ab137838 diluted at 1/1000.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab137838 被引用在 36 文献中.
- Ren J et al. Somatic variants of MAP3K3 are sufficient to cause cerebral and spinal cord cavernous malformations. Brain 146:3634-3647 (2023). PubMed: 36995941
- Jin QW et al. ROP16 of Toxoplasma gondii Inhibits Innate Immunity by Triggering cGAS-STING Pathway Inactivity through the Polyubiquitination of STING. Cells 12:N/A (2023). PubMed: 37508526
- Tian R et al. RACK1 facilitates breast cancer progression by competitively inhibiting the binding of β-catenin to PSMD2 and enhancing the stability of β-catenin. Cell Death Dis 14:685 (2023). PubMed: 37848434
- Tuzlakoğlu Öztürk M & Güllülü Ö Dimerization underlies the aggregation propensity of intrinsically disordered coiled-coil domain-containing 124. Proteins 90:218-228 (2022). PubMed: 34369007
- Vlahos AE et al. Protease-controlled secretion and display of intercellular signals. Nat Commun 13:912 (2022). PubMed: 35177637