Anti-HA tag抗体[12CA5] (ab1424)
![Western blot - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.cn/ps/products/1/ab1424/Images/HA-tag-Primary-antibodies-ab1424-6.jpg "Western blot - Anti-HA tag antibody [12CA5] (ab1424)")
Key features and details
- Mouse monoclonal [12CA5] to HA tag
- Suitable for: ICC/IF, ChIP, IP, WB
- Reacts with: Species independent
- Isotype: IgG2b
概述
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产品名称
Anti-HA tag抗体[12CA5]
参阅全部 HA tag 一抗 -
描述
小鼠单克隆抗体[12CA5] to HA tag -
宿主
Mouse -
特异性
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data). -
经测试应用
适用于: ICC/IF, ChIP, IP, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. Derived from the human influenza hemaglutinin protein
Database link: ADG21028 -
阳性对照
- WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3996 HA-Tag Antibody (Clone 12CA5[2]).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.03% Proclin 300
Constituents: 30% Glycerol (glycerin, glycerine), 0.05% BSA, PBS, 0.019% Ethylenediaminetetraacetic acid, disodium, dihydrate -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
12CA5 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| ChIP |
Use a concentration of 3 µg/ml.
Use 3 µg per 25 µg chromatin (see figure legend). |
|
| IP | (5) |
Use a concentration of 10 µg/ml.
|
| WB | (6) |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
ChIP
Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend). |
|
IP
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Western blot - Anti-HA tag antibody [12CA5] (ab1424)This image is courtesy of an anonymous AbreviewAnti-HA tag antibody [12CA5] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
Secondary
HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 seconds
Blocked with 5% milk for 30 min at 25°C -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.cn/ps/products/1/ab1424/Images/ab1424_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)This image was kindly supplied as part of the review submitted by one of our customers.This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
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![Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.cn/ps/products/1/ab1424/Images/ab1424-302929-1424.jpg)
Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)Data courtesy of an anonymous AbReviewRecombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
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![ChIP - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.cn/ps/products/1/ab1424/Images/ab1424_2.jpg)
ChIP - Anti-HA tag antibody [12CA5] (ab1424)Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab1424 被引用在 45 文献中.
- Melendez J et al. TGFß signalling acts as a molecular brake of myoblast fusion. Nat Commun 12:749 (2021). PubMed: 33531476
- Grigsby SM et al. Elucidating the Importance of DOT1L Recruitment in MLL-AF9 Leukemia and Hematopoiesis. Cancers (Basel) 13:N/A (2021). PubMed: 33562706
- Lei G et al. TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. Can J Gastroenterol Hepatol 2021:8870907 (2021). PubMed: 33824865
- Shorstova T et al. Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition. Mol Cancer Ther 20:64-75 (2021). PubMed: 33087508
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910