AB108540

重组Anti-H2R抗体[EPR5630]

Anti-H2R antibody [EPR5630]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal H2R antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2

1 Images

Unknown

Western blot - Anti-H2R antibody [EPR5630] (AB108540)

All lanes:

Western blot - Anti-H2R antibody [EPR5630] (ab108540) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Human adrenal gland lysate at 10 µg

Predicted band size: 40 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5630

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

H2R also known as histamine H2 receptor is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is expressed mainly in the gastric parietal cells as well as in the heart brain and various immune cells. H2R modulates gastric acid secretion by engaging in the interaction with histamine which activates the receptor. This activation includes changes in intracellular cyclic AMP levels and subsequent cellular responses.

Biological function summary

The histamine H2 receptor plays a significant role in the mediation of gastric acid secretion cardiac muscle contraction and immune response modulation. Although it functions independently it does interact with other receptors giving a context like mast cells which secrete histamine. These receptors play an important role in stimulating gastric parietal cells to produce acid as well as having involvement in the modulation of heart rate and force of contraction.

Pathways

H2R participates in the GPCR signaling pathway and adenylate cyclase activation both of which are critical for physiological responses in gastric acid secretion and modulation of cardiac function. H2R interacts with proteins such as adenylate cyclase and other H receptors like H1 receptor contributing to the regulation of varied responses across tissues like the stomach heart and brain.

H2R is strongly related to peptic ulcers and gastroesophageal reflux disease (GERD). In these conditions excessive secretion of gastric acid due to H2R overactivity can lead to mucosal damage and discomfort. Moreover other histamine receptors like H1 receptor might influence inflammatory processes and allergy drawing connections that are relevant to understanding histamine's overall impacts in the body.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).

See full target information Histamine H2 receptor

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1312 PubMed40885864

2025

A single-cell atlas of mouse central nervous system immune cells reveals unique infection-stage immune signatures during the progression of meningitis caused by Streptococcus suis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Jiang,Jikun Mei,Junhui Zhu,Yanyan Tian,Tong Wu,Ziheng Li,Zengshuai Wu,Tamim Abdelaal,Fengyang Li,Na Li,Liancheng Lei

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-H2R抗体[EPR5630]

Anti-H2R antibody [EPR5630]

Western blot - Anti-H2R antibody [EPR5630] (AB108540)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原