Anti-GW182抗体(ab114857)
Key features and details
- Rabbit polyclonal to GW182
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GW182抗体 -
描述
兔多克隆抗体to GW182 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1225-1275 of Human GW182 (NP_055309.2).
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阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114857于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 10 µg/mg of lysate.
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说明 |
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IP
Use at 10 µg/mg of lysate. |
靶标
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功能
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain. -
细胞定位
Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 27327 Human
- Entrez Gene: 233833 Mouse
- Entrez Gene: 308971 Rat
- Omim: 610739 Human
- SwissProt: Q8NDV7 Human
- SwissProt: Q3UHK8 Mouse
- Unigene: 655057 Human
- Unigene: 102305 Mouse
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别名
- CAG repeat protein 26 antibody
- CAGH26 antibody
- DKFZp666E117 antibody
see all
图片
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Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab114857 尚未被引用在任何文献中。