Anti-GTF3C5抗体(ab74305)
Key features and details
- Rabbit polyclonal to GTF3C5
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GTF3C5抗体 -
描述
兔多克隆抗体to GTF3C5 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide from a region between residue 469 and 519 of human GTF3C5 (using the numbering given in entry NP_036219.1)
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阳性对照
- Lysate from HeLa, 293T or NIH3T3 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters. -
序列相似性
Belongs to the TFIIIC subunit 5 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 417164 Chicken
- Entrez Gene: 464820 Chimpanzee
- Entrez Gene: 101143035 Gorilla
- Entrez Gene: 9328 Human
- Entrez Gene: 70239 Mouse
- Omim: 604890 Human
- SwissProt: Q5T7U2 Human
- SwissProt: Q5T7U3 Human
see all -
别名
- FLJ20857 antibody
- General transcription factor 3C polypeptide 5 antibody
- General transcription factor IIIC polypeptide 5 63kDa antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF3C5 antibody (ab74305)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
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Western blot - Anti-GTF3C5 antibody (ab74305)All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-GTF3C5 antibody (ab74305)20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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