Anti-gtf3c3抗体(ab74230)
Key features and details
- Rabbit polyclonal to gtf3c3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-gtf3c3抗体 -
描述
兔多克隆抗体to gtf3c3 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)
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阳性对照
- IP: HEK293T lysate; WB: HeLa, Jurkat cell lysates; IHC: human breast carcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74230于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 101 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters. -
序列相似性
Contains 11 TPR repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9330 Human
- Entrez Gene: 316810 Rat
- Omim: 604888 Human
- SwissProt: Q9Y5Q9 Human
- Unigene: 470996 Human
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别名
- General transcription factor 3C polypeptide 3 antibody
- general transcription factor IIIC, polypeptide 3 (102kD) antibody
- general transcription factor IIIC, polypeptide 3, 102kD antibody
see all
图片
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Western blot - Anti-gtf3c3 antibody (ab74230)All lanes : Anti-gtf3c3 antibody (ab74230) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 101 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)gtf3c3 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab74230 at 3 µg per reaction. Western blot was performed on the immunoprecipitate using ab74230 at 1 µg/mL.
Lane 1: ab74230 IP in HEK293T whole cell lysate.
Lane 2: ab74230 IP in HEK293T whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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