Anti-GTF2H1抗体(ab232982)
Key features and details
- Rabbit polyclonal to GTF2H1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-GTF2H1抗体
参阅全部 GTF2H1 一抗 -
描述
兔多克隆抗体to GTF2H1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse
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免疫原
Recombinant fragment (His-T7-tag) corresponding to Human GTF2H1 aa 50-350. N-terminal tags. Expressed in E. coli.
Database link: P32780 -
阳性对照
- IHC-P: Human liver tissue. WB: Pig cerebrum and liver tissue lysate. HepG2 cell lysate. Recombinant human GTF2H1 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232982于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa.
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| IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa. |
|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. -
序列相似性
Contains 2 BSD domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2965 Human
- Entrez Gene: 14884 Mouse
- Omim: 189972 Human
- SwissProt: P32780 Human
- SwissProt: Q9DBA9 Mouse
- Unigene: 577202 Human
- Unigene: 22700 Mouse
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别名
- Basic transcription factor 2 62 kDa subunit antibody
- Basic transcription factor 62 kDa subunit antibody
- BTF 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (ab232982)
Formalin-fixed, paraffin-embedded human liver tissue stained for GTF2H1 with ab232982 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-GTF2H1 antibody (ab232982)Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + Pig cerebrum tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Western blot - Anti-GTF2H1 antibody (ab232982)Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Western blot - Anti-GTF2H1 antibody (ab232982)Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + Pig liver tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Western blot - Anti-GTF2H1 antibody (ab232982)Anti-GTF2H1 antibody (ab232982) at 2 µg/ml + Recombinant human GTF2H1 protein
Predicted band size: 62 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab232982 被引用在 1 文献中.
- Haase J et al. The TFIIH complex is required to establish and maintain mitotic chromosome structure. Elife 11:N/A (2022). PubMed: 35293859