AB248238

重组Anti-GTF2F2抗体[EPR7584] - BSA and Azide free

Anti-GTF2F2 antibody [EPR7584] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GTF2F2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

RAP30, GTF2F2, General transcription factor IIF subunit 2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, Transcription initiation factor RAP30, TFIIF-beta

1 Images

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Western blot - Anti-GTF2F2 antibody [EPR7584] - BSA and Azide free (AB248238)

This data was developed using ab128945, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GTF2F2 antibody [EPR7584] (<a href='/products/primary-antibodies/gtf2f2-antibody-epr7584-ab128945'>ab128945</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 30 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-GTF2F2 antibody [EPR7584]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7584

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab248238 is the carrier-free version of ab128945.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GTF2F2 also known as RAP74 forms a critical component of the general transcription factor IIF. It possesses an approximate mass of 74 kDa. This protein ubiquitously expresses in human cells acting as a cofactor within RNA polymerase II transcription machinery. GTF2F2 binds with RNA polymerase II influencing transcription initiation by stabilizing the transcription pre-initiation complex.

Biological function summary

GTF2F2 plays a significant role in enhancing the process of transcription elongation. It joins the multi-protein transcription factor IIF complex collaborating with subunits such as TFIIF. GTF2F2's activity is necessary for promoter clearance and efficient RNA synthesis from DNA templates bridging interactions between transcription factors and the polymerase.

Pathways

Involvement of GTF2F2 is important in the transcriptional regulation pathway. It coordinates with RNA polymerase II and influences the transcription cycle particularly pathways related to transcription elongation. GTF2F2 shares interactions with other transcription-associated proteins including TFIIB and TFIIH which further integrate into these pathways to optimize transcription efficiency and fidelity.

Studies link GTF2F2 to neurological disorders including mental retardation. The protein's disruption might alter transcriptional regulation leading to developmental anomalies. In cancer it holds a connection with proteins like p53 where changes in transcription factor activity influenced by GTF2F2 can affect cancer progression or response to treatment through disruption of proper gene expression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.

See full target information GTF2F2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20220613 PubMed36694627

2023

circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Chunping Yang,Ping Li

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-GTF2F2抗体[EPR7584] - BSA and Azide free