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Anti-GSTT1抗体(ab175418)

Submit a review Q&A (1)References (1)
Western blot - Anti-GSTT1 antibody (ab175418)

    Key features and details

    • Rabbit polyclonal to GSTT1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-GSTT1 antibody [EPR19283] (ab199337)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-GSTT1抗体
      参阅全部 GSTT1 一抗

    • 描述

      兔多克隆抗体to GSTT1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Cow

    • 免疫原

      Recombinant full length protein corresponding to Human GSTT1 aa 1-240.
      Sequence:

      MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDAFAQVNP LKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLA WQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDK FLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEA AVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR


      Database link: P30711
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.30
      Preservative: 0.09% Sodium azide
      Constituents: 49.91% PBS, 50% Glycerol (glycerin, glycerine)
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab175418于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/2000. Predicted molecular weight: 27 kDa.
    说明
    WB
    1/500 - 1/2000. Predicted molecular weight: 27 kDa.

    靶标

    • 功能

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

    • 组织特异性

      Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

    • 序列相似性

      Belongs to the GST superfamily. Theta family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 数据库链接

      • 别名

        • EC 2.5.1.18 antibody
        • Glutathione S transferase 5 antibody
        • Glutathione S transferase theta 1 antibody
        • Glutathione S-transferase theta-1 antibody
        • Glutathione transferase T1 1 antibody
        • Glutathione transferase T1-1 antibody
        • GST 5 5 antibody
        • GST CL1 antibody
        • GST class theta 1 antibody
        • GST class-theta-1 antibody
        • GSTT1 antibody
        • GSTT1_HUMAN antibody
        see all

      图片

      • Western blot - Anti-GSTT1 antibody (ab175418)
        Western blot - Anti-GSTT1 antibody (ab175418)
        Anti-GSTT1 antibody (ab175418) at 1/500 dilution + HepG2 cell extract

        Predicted band size: 27 kDa

      文献 (1)

      发表研究结果有使用 ab175418？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab175418 被引用在 1 文献中.

      • Xie MY  et al. 5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina. Int J Ophthalmol 12:1-7 (2019). PubMed: 30662833

      客户评价及客户问答

      Show All 评价 Q&A
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      Question

      Hello! I am looking to buy a human GSTT1 primary antibody for western blotting. I wish to avoid cross-reactivity with GSTT2 and am looking for an antibody that preferably binds to the C-terminus.

      Read More
      Answer

      The Anti-Glutathione S Transferase theta 1 antibody (ab81033) has not been tested for cross-reactivity with the GSTT2 protein. The short immunogen peptide (˜14 aa) used to raise this antibody has 100% homology to human GSTT1 protein. The immunogen peptide has no homology/ similarity to the human GSTT2 protein, therefore it is safe to say that it will not be cross-reactive.

      Unfortunately, the Anti-Glutathione S Transferase theta 1 antibody (ab96592) has not been tested in any cross-reactivity experiment to determine the specificity of the antibody for GSTT1. The homology identity of the ab96592 immunogen to GSTT2 is 54%. Therefore there is a chance that the antibody would react with GSTT2.

      Unfortunately, the Anti-Glutathione S Transferase theta 1 (ab175418) antibody has not been tested in any cross-reactivity experiment to determine the specificity of the antibody for GSTT1. The molecular weight of these two protein are closed therefore the two proteins cannot be distinguished through WB experiment.

      Read More

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