重组Anti-GSTK1抗体[EPR1939] (ab134173)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1939] to GSTK1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GSTK1抗体[EPR1939]
参阅全部 GSTK1 一抗 -
描述
兔单克隆抗体[EPR1939] to GSTK1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human GSTK1 aa 1-100. The exact sequence is proprietary.
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阳性对照
- WB: Daudi, HeLa and HEK-293 cell lysates IHC-P Human kidney tissue ICC/IF: HEK-293 cells Flow Cyt (intra): HeLa Cells IP: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1939 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. |
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WB |
1/1000. Predicted molecular weight: 25 kDa.
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IP |
1/30.
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ICC/IF |
1/50.
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说明 |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. |
WB
1/1000. Predicted molecular weight: 25 kDa. |
IP
1/30. |
ICC/IF
1/50. |
靶标
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功能
Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the GST superfamily. Kappa family. -
细胞定位
Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 373156 Human
- Omim: 602321 Human
- SwissProt: Q9Y2Q3 Human
- Unigene: 390667 Human
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别名
- EC 2.5.1.18 antibody
- Glutathione S Transferase kappa 1 antibody
- Glutathione S transferase subunit 13 antibody
see all
图片
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All lanes : Anti-GSTK1 antibody [EPR1939] (ab134173) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTK1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab134173 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab134173 Anti-GSTK1 antibody [EPR1939] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used. Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE. ab134173 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-GSTK1 antibody [EPR1939] (ab134173) at 1/1000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling GSTK1 with purified ab134173 at 1/300 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling GSTK1 with Purified ab134173 at 1/50 dilution (5.92 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Purified ab134173 at 1/30 dilution (2ug) immunoprecipitating GSTK1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (10µg)
Lane 2 (+): ab134173 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134173 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa -
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GSTK1 with Purified ab134173 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134173 被引用在 1 文献中.
- Zeng F et al. Glutathione S-transferase kappa 1 is positively related with sperm quality of porcine sperm. Mol Reprod Dev 89:104-112 (2022). PubMed: 34888969