AB9085

Anti-GST 抗体

Name: GST抗体 (ab9085)| Abcam中文官网
Brand: Abcam Limited
SKU: AB9085
Price: 3959 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-GST antibody

Lab Essentials
了解详情

(2 Reviews)

(74 Publications)

Rabbit Polyclonal GST antibody. Suitable for WB and reacts with Tag samples. Cited in 74 publications.

查看别名

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

AbReview30171****

Western blot - Anti-GST antibody (AB9085)

Blocked with 5% Milk for 1 hour at 25°C.

All lanes:

Western blot - Anti-GST antibody (ab9085) at 1/2000 dilution

Lane 1:

Recombinant GST protein purified from E. coli XL1-Blue at 10 µg

Lane 2:

Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue at 10 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa,80 kDa

true

Exposure time: 5s

This image is courtesy of an anonymous Abreview

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

应用

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Affinity purified against bacterially expressed GST

存储溶液

pH: 7 Constituents: 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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文献 (74)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:31643 PubMed40866490

2025

RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Lital Estrella Weil,Michal Feldman,Jennifer Van Duine,Ji Qiu,Joshua LaBaer,Dan Levy

Molecular metabolism 84:101938 PubMed38631478

2024

Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie J Desmet,Jonathan Thommis,Tineke Vanderhaeghen,Edmee M F Vandenboorn,Dorien Clarisse,Yunkun Li,Steven Timmermans,Daria Fijalkowska,Dariusz Ratman,Evelien Van Hamme,Lode De Cauwer,Bart Staels,Luc Brunsveld,Frank Peelman,Claude Libert,Jan Tavernier,Karolien De Bosscher

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308945 PubMed38627980

2024

Loss of OVOL2 in Triple-Negative Breast Cancer Promotes Fatty Acid Oxidation Fueling Stemness Characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Ruipeng Lu,Jingjing Hong,Tong Fu,Yu Zhu,Ruiqi Tong,Di Ai,Shuai Wang,Qingsong Huang,Ceshi Chen,Zhiming Zhang,Rui Zhang,Huiling Guo,Boan Li

Redox biology 72:103150 PubMed38599016

2024

S-Adenosyl-l-methionine restores brain mitochondrial membrane fluidity and GSH content improving Niemann-Pick type C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Leire Goicoechea,Sandra Torres,Laura Fàbrega,Mónica Barrios,Susana Núñez,Josefina Casas,Gemma Fabrias,Carmen García-Ruiz,José C Fernández-Checa

The Journal of clinical investigation 133: PubMed37643007

2023

The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Jie Chen,Caihua Zhang,Maosheng Cheng,Shuang Chen,Wei Song,Chunlong Yang,Rongsong Ling,Zhi Chen,Xiaochen Wang,Gan Xiong,Jieyi Ma,Yan Zhu,Quan Yuan,Qi Liu,Liang Peng,Qianming Chen,Demeng Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23198 PubMed37742307

2023

DOCK3 regulates normal skeletal muscle regeneration and glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne Samani,Muthukumar Karuppasamy,Katherine G English,Colin A Siler,Yimin Wang,Jeffrey J Widrick,Matthew S Alexander

The Journal of biological chemistry 299:105236 PubMed37690684

2023

Methylation of the transcription factor E2F1 by SETD6 regulates SETD6 expression via a positive feedback mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Kublanovsky,Gizem T Ulu,Sara Weirich,Nurit Levy,Michal Feldman,Albert Jeltsch,Dan Levy

Nature communications 14:5194 PubMed37626056

2023

Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.

Applications

Unspecified application

Species

Unspecified reactive species

Cunmin Qu,Meichen Zhu,Ran Hu,Yongchao Niu,Si Chen,Huiyan Zhao,Chengxiang Li,Zhen Wang,Nengwen Yin,Fujun Sun,Zhiyou Chen,Shulin Shen,Guoxia Shang,Yan Zhou,Xingying Yan,Lijuan Wei,Liezhao Liu,Bin Yi,Jinmin Lian,Jiang Li,Zhanglin Tang,Ying Liang,Xinfu Xu,Rui Wang,Jiaming Yin,Huafang Wan,Hai Du,Wei Qian,Yourong Chai,Qingyuan Zhou,Yajun He,Silin Zhong,Xiao Qiu,Hao Yu,Hon-Ming Lam,Kun Lu,Fuyou Fu,Jiana Li

The EMBO journal 42:e113349 PubMed37306101

2023

Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Ikeda,Daisuke Noshiro,Hideaki Morishita,Shuhei Takada,Shun Kageyama,Yuko Fujioka,Tomoko Funakoshi,Satoko Komatsu-Hirota,Ritsuko Arai,Elena Ryzhii,Manabu Abe,Tomoaki Koga,Hozumi Motohashi,Mitsuyoshi Nakao,Kenji Sakimura,Arata Horii,Satoshi Waguri,Yoshinobu Ichimura,Nobuo N Noda,Masaaki Komatsu

The New phytologist 238:1908-1923 PubMed36882897

2023

BBX30/miP1b and BBX31/miP1a form a positive feedback loop with ABI5 to regulate ABA-mediated postgermination seedling growth arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Deeksha Singh,Sourav Datta

View all publications

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Anti-GST 抗体

Anti-GST antibody

Western blot - Anti-GST antibody (AB9085)