Anti-GST抗体[3G10/1B3] (ab92)
Key features and details
- Mouse monoclonal [3G10/1B3] to GST
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-GST抗体[3G10/1B3]
参阅全部 GST 一抗 -
描述
小鼠单克隆抗体[3G10/1B3] to GST -
宿主
Mouse -
特异性
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum. -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
Database link: P08515 -
常规说明
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS
w/o preservative -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3G10/1B3 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
|
IP |
Use at an assay dependent dilution.
|
说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
IP
Use at an assay dependent dilution. |
靶标
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相关性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
别名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
图片
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All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution
Lane 1 : Non-induced GST-fusion protein expressed in E.coli
Lane 2 : IPTG induced GST-fusion protein expressed in E.coli
Lysates/proteins at 5 µl per lane.
Predicted band size: 26 kDa10% SDS-PAGE
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All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size: 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
数据表及文件
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Datasheet download
文献 (24)
ab92 被引用在 24 文献中.
- Zhang X et al. PTPN22 interacts with EB1 to regulate T-cell receptor signaling. FASEB J 34:8959-8974 (2020). PubMed: 32469452
- Marquardt J et al. The LKB1-like Kinase Elm1 Controls Septin Hourglass Assembly and Stability by Regulating Filament Pairing. Curr Biol 30:2386-2394.e4 (2020). PubMed: 32386534
- Geymonat M et al. Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase. Elife 9:N/A (2020). PubMed: 32851976
- Pantier R et al. TET1 Interacts Directly with NANOG via Independent Domains Containing Hydrophobic and Aromatic Residues. J Mol Biol 432:6075-6091 (2020). PubMed: 33058869
- He Y et al. The OsGSK2 Kinase Integrates Brassinosteroid and Jasmonic Acid Signaling by Interacting with OsJAZ4. Plant Cell 32:2806-2822 (2020). PubMed: 32586913