AB92

Anti-GST抗体[3G10/1B3]

Name: GST抗体[3G10/1B3] (ab92)| Abcam中文官网
Brand: Abcam Limited
SKU: AB92
Price: 3959 CNY
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Anti-GST antibody [3G10/1B3]

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(3 Reviews)

(42 Publications)

Mouse Monoclonal GST antibody. Carrier free. Suitable for IP, WB and reacts with Tag samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

查看别名

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

4 Images

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA2 antibody [EPR22919-71] - ChIP Grade ab256493 staining at 1/1000 dilution, shown in green; anti-GST antibody [3G10/1B3] ab92 staining at 1 g/mL, shown in magenta. A band was observed at 78 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA2 antibody [EPR22919-71] - ChIP Grade (<a href='/products/primary-antibodies/foxa2-antibody-epr22919-71-chip-grade-ab256493'>ab256493</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA2 antibody [EPR4466] ab108422 staining at 1/1000 dilution, shown in green; [LOADING CONTROL] loading control staining at 1/[DILUTION] dilution, shown in magenta. A band was observed at 78 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA2 antibody [EPR4466] (<a href='/products/primary-antibodies/foxa2-antibody-epr4466-ab108422'>ab108422</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA1 antibody ab23738 staining at 0.7 µg/mL, shown in green; Mouse anti GST ab92 loading control staining at 1/1000 dilution, shown in magenta. A band was observed at 76 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 antibody (<a href='/products/primary-antibodies/foxa1-antibody-ab23738'>ab23738</a>) at 0.7 µg/mL

Lane 1:

FOXA1 Recombinant Protein at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

false

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Anti-FOXA1 + FOXA2 + FOXA3 antibody [RM1119] ab317046 staining at 1/1000 dilution, shown in green; Mouse anti GST (ab92) loading control staining at 1/200 dilution, shown in magenta. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 + FOXA3 antibody [RM1119] (<a href='/products/primary-antibodies/foxa1-antibody-rm1119-ab317046'>ab317046</a>) at 1/1000 dilution

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa,50 kDa,37 kDa

Observed band size: 78 kDa,76 kDa,63 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

3G10/1B3

亚型

IgG1

轻链类型

kappa

不含载体蛋白

Yes

应用

IP, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

P08515

特异性

Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.

style

left-column