重组Anti-GSK3 beta (phospho S9)抗体[EPR2286Y] (ab75814)
![Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-255845-anti-gsk3-beta-phospho-s9-antibody-epr2286y-western-blot.jpg "Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2286Y] to GSK3 beta (phospho S9)
- Suitable for: Dot blot, WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GSK3 beta (phospho S9)抗体[EPR2286Y]
参阅全部 GSK3 beta 一抗 -
描述
兔单克隆抗体[EPR2286Y] to GSK3 beta (phospho S9) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T cell lysates, untreated or treated with Calyculin A and human ovarian carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2286Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
|
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| WB | (7) |
1/10000 - 1/20000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (2) |
1/100 - 1/250.
|
| 说明 |
|---|
|
Dot blot
Use at an assay dependent concentration. |
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WB
1/10000 - 1/20000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
靶标
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功能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
组织特异性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2932 Human
- Omim: 605004 Human
- SwissProt: P49841 Human
- Unigene: 445733 Human
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别名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
图片
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Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)All lanes : Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814) at 1/20000 dilution
Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cells were starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes.
Lane 3 : HeLa cells were starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes. The membrane was treated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat anti-rabbit IgG H&L (HRP))
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking buffer: 5% NFDM/TBST for the first 3 lanes, 2% BSA/TBST for the second 3 lanes.
Dilution buffer: 5% NFDM/TBST for the first 3 lanes, 2% BSA/TBST for the second 3 lanes.
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![Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-256147-anti-gsk3-beta-phospho-s9-antibody-epr2286y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)Cell line: HeLa (human cervix adenocarcinoma)
Target: Ab75814 anti-GSK3 beta (phospho S9)
Secondary: ab150077 AlexaFluor®488 Goat anti-Rabbit
Fixative: 4% PFA
Permeabilization: 0.1% Triton-X
Nuclear counter stain: DAPI
Confocal image showed increased cytoplasmic staining after Calyculin A (CA) (ab141784 100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA.
Ab32391 was used as a control for ab75814. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-256032-anti-gsk3-beta-phospho-s9-antibody-epr2286y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)Immunohistochemical analysis of paraffin-embedded human breast tissue labelled with untreated ab75814 (phospho) (top-left) at a dilution of 1/1000, alkaline phosphatase treated ab75814 (phospho) (top-right) at a dilution of 1/1000, untreated ab32391 (bottom-left) at a dilution of 1/1000 and alkaline phophatase treated ab32391 (bottom-right) at a dilution of 1/1000. Ab97051 was used as secondary antibody at a dilution of 1/500 and counterstained with hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Dot Blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-251035-anti-gsk3-beta-phospho-s9-antibody-epr2286y-dot-blot.jpg)
Dot Blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)Dot blot analysis of GSK3 beta (pS9) peptide (Lane 1) and GSK3 beta non-phospho peptide (Lane 2) labelling GSK3 (pS9) with purified ab75814 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-256033-anti-gsk3-beta-phospho-s9-antibody-epr2286y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelled with untreated ab75814 (top-left) at a dilution of 1/1000, alkaline phosphatase treated ab75814 (top-right) at a dilution of 1/1000, untreated ab32391 (bottom-left) at a dilution of 1/1000 and alkaline phophatase treated ab32391 (bottom-right) at a dilution of 1/1000. Ab97051 was used as secondary antibody at a dilution of 1/500 and counterstained with hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-90909-anti-gsk3-beta-phospho-s9-antibody-epr2286y-western-blot.gif)
Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)All lanes : Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814) at 1/20000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
![Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)](https://www.abcam.cn/ps/products/75/ab75814/Images/ab75814-9-benefits-of-recombinant-antibodies.png)
Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (111)
ab75814 被引用在 111 文献中.
- Gao J et al. Melatonin enhances the sensitivity of colorectal cancer cells to 5-fluorouracil through the regulation of the miR-532-3p/β-catenin pathway. Environ Toxicol 39:367-376 (2024). PubMed: 37755321
- Huang M et al. CEP55 as a promising biomarker and therapeutic target on gallbladder cancer. Front Oncol 13:1156177 (2023). PubMed: 37274251
- Balamurugan K et al. Stabilization of E-cadherin adhesions by COX-2/GSK3β signaling is a targetable pathway in metastatic breast cancer. JCI Insight 8:N/A (2023). PubMed: 36757813
- Liu Y et al. Utilizing network pharmacology and experimental validation to explore the potential molecular mechanisms of raw Pinellia ternate in treating esophageal cancer. J Gastrointest Oncol 14:2006-2017 (2023). PubMed: 37969842
- Tian L et al. C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages. iScience 26:107771 (2023). PubMed: 37731607