Anti-GSK3 beta (phospho S9)抗体(ab131097)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho S9)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GSK3 beta (phospho S9)抗体
参阅全部 GSK3 beta 一抗 -
描述
兔多克隆抗体to GSK3 beta (phospho S9) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293 cell extract treated with serum; HeLa cells; Human breast carcinoma and lung carcinoma tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131097 was purified by affinity chromatography using epitope specific phosphopetide. Non phospho specific antibodies were removed by chromatography using non phosphopetide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131097于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 46 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
组织特异性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2932 Human
- Omim: 605004 Human
- SwissProt: P49841 Human
- Unigene: 445733 Human
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别名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
图片
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All lanes : Anti-GSK3 beta (phospho S9) antibody (ab131097) at 1/500 dilution
Lane 1 : 293 cell extract, untreated
Lane 2 : 293 cell extract treated with serum
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody (ab131097)Immunohistochemical staining of GSK3 beta (phospho S9) in paraffin embedded Human breast carcinoma tissue, using ab131097 at a 1/50 dilution.
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Immunofluorescence analysis of methanol fixed HeLa cells staining GSK3 beta (phospho S9) with ab131097 at a dilution of 1/100.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSK3 beta (phospho S9) (red) using ab131097 at 1/100 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody (ab131097)
Paraffin-embedded human lung carcinoma tissue stained for GSK3 beta (phospho S9) using ab131097 at 1/50 dilution in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (78)
ab131097 被引用在 78 文献中.
- Wu Y et al. Renalase Prevents Renal Fibrosis by Inhibiting Endoplasmic Reticulum Stress and Down-Regulating GSK-3β/Snail Signaling. Int J Med Sci 20:669-681 (2023). PubMed: 37082730
- Li LF et al. Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway. Phytother Res 37:4838-4850 (2023). PubMed: 37458182
- Sun Y et al. CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo. J Gene Med 24:e3343 (2022). PubMed: 33871149
- Silva-Aguiar RP et al. Albumin Expands Albumin Reabsorption Capacity in Proximal Tubule Epithelial Cells through a Positive Feedback Loop between AKT and Megalin. Int J Mol Sci 23:N/A (2022). PubMed: 35055044
- Wang P et al. MicroRNA-1246 suppresses the metastasis of breast cancer cells by targeting the DYRK1A/PGRN axis to prevent the epithelial-mesenchymal transition. Mol Biol Rep 49:2711-2721 (2022). PubMed: 35059968