Anti-GSK3 alpha (phospho S21)抗体(ab28808)
Key features and details
- Rabbit polyclonal to GSK3 alpha (phospho S21)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GSK3 alpha (phospho S21)抗体
参阅全部 GSK3 alpha 一抗 -
描述
兔多克隆抗体to GSK3 alpha (phospho S21) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to GSK3 alpha (phospho S21).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28808于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 2931 Human
- Entrez Gene: 50686 Rat
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: P18265 Rat
- Unigene: 466828 Human
- Unigene: 36807 Rat
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别名
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
see all
图片
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Western blot - Anti-GSK3 alpha (phospho S21) antibody (ab28808)All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution
Lane 1 : Extracts from ovary cancer
cells.
Lane 2 : Extracts from ovary cancer
cells. with synthesised non phosphopeptide at 1 µg/ml
Lane 3 : Extracts from ovary cancer
cells. with synthesised phosphopeptide at 1 µg/ml
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha (phospho S21) antibody (ab28808)Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha (phospho S21) antibody (ab28808)ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab28808 被引用在 6 文献中.
- Sun F et al. Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3ß/ß-catenin signaling pathway. Aging (Albany NY) 13:22528-22543 (2021). PubMed: 34555811
- Zhang Y et al. Integrated High Throughput Analysis Identifies GSK3 as a Crucial Determinant of p53-Mediated Apoptosis in Lung Cancer Cells. Cell Physiol Biochem 42:1177-1191 (2017). PubMed: 28662516
- Qian CJ et al. Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells. Oncol Lett 12:3499-3505 (2016). WB ; Human . PubMed: 27900027
- Borghgraef P et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS One 8:e84442 (2013). WB ; Mouse . PubMed: 24376810
- Yamaguchi N et al. Dysfunctional ryanodine receptor and cardiac hypertrophy: role of signaling molecules. Am J Physiol Heart Circ Physiol 300:H2187-95 (2011). WB ; Mouse . PubMed: 21421818
- Kapfhamer D et al. Protein Phosphatase 2a and glycogen synthase kinase 3 signaling modulate prepulse inhibition of the acoustic startle response by altering cortical M-Type potassium channel activity. J Neurosci 30:8830-40 (2010). WB ; Mouse . PubMed: 20592205