Anti-GSK3 (alpha + beta) (phospho Y216 + Y279)抗体[M132] (ab45383)
Key features and details
- Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279)
- Suitable for: WB
- Reacts with: Rabbit
- Isotype: IgG1
概述
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产品名称
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279)抗体[M132]
参阅全部 GSK3 (alpha + beta) 一抗 -
描述
小鼠单克隆抗体[M132] to GSK3 (alpha + beta) (phospho Y216 + Y279) -
宿主
Mouse -
特异性
This antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3ß and GSK-3a -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rabbit
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human GSK3 (alpha + beta) (phospho Y216) conjugated to keyhole limpet haemocyanin.
Database link: P49841 -
阳性对照
- Pervanadate treated rabbit fibroblasts and treated human SKN-SH and A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M132 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45383于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 52 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 52 kDa. |
靶标
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相关性
Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- SwissProt: Q2NL51 Mouse
- SwissProt: Q9WV60 Mouse
- SwissProt: P18265 Rat
- SwissProt: P18266 Rat
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别名
- Factor A antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase 3 beta antibody
see all
图片
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Lanes 1-2 : anti-GSK-3ß
Lanes 3-4 : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383) at 1/1000 dilution
Lanes 1 & 3 : rabbit spleen fibroblasts serum starved for 2 hrs
Lanes 2 & 4 : rabbit spleen fibroblasts treated with pervanadate (1 mM) for 30 minutes
Predicted band size: 52 kDa
Observed band size: 46,50 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab45383 被引用在 1 文献中.
- Puri P et al. Proteomic Analysis of Bovine Sperm YWHA Binding Partners Identify Proteins Involved in Signaling and Metabolism. Biol Reprod : (2008). WB ; Cow . PubMed: 18753613