重组Anti-GSDMC抗体[EPR20887-50] (ab225635)
![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)](https://www.abcam.cn/ps/products/225/ab225635/Images/ab225635-412155-anti-gsdmc1-antibody-epr2088750-western-blot-lysate.jpg "Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20887-50] to GSDMC
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-GSDMC抗体[EPR20887-50]
参阅全部 GSDMC 一抗 -
描述
兔单克隆抗体[EPR20887-50] to GSDMC -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse stomach and esophagus tissue lysates; HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20887-50 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 52, 54 kDa (predicted molecular weight: 53 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 52, 54 kDa (predicted molecular weight: 53 kDa). |
靶标
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组织特异性
Expressed primarily in trachea and spleen. -
序列相似性
Belongs to the gasdermin family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 83492 Mouse
- SwissProt: Q99NB5 Mouse
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别名
- Gasdermin C antibody
- Gasdermin-C antibody
- GSDMC antibody
see all
图片
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Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse esophagus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds Lane 2: 3.25 seconds.
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![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)](https://www.abcam.cn/ps/products/225/ab225635/Images/ab225635-412156-anti-gsdmc1-antibody-epr2088750-western-blot-tissue.jpg)
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : Wild-type Mouse esophagus tissue lysate
Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
Lane 3 : Wild-type Mouse stomach tissue lysate
Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
Lane 5 : Wild-type Mouse small intestine tissue lysate
Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
Lane 7 : Wild-type Mouse colon tissue lysate
Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
Lane 9 : Wild-type Mouse caecum tissue lysate
Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
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![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)](https://www.abcam.cn/ps/products/225/ab225635/Images/ab225635-412157-anti-gsdmc1-antibody-epr2088750-western-blot-kidney.jpg)
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
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![Anti-GSDMC antibody [EPR20887-50] (ab225635)](https://www.abcam.cn/ps/products/225/ab225635/Images/ab225635-4-benefits-of-recombinant-antibodies.png)
Anti-GSDMC antibody [EPR20887-50] (ab225635)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab225635 尚未被引用在任何文献中。