AB247751

重组Anti-GSC抗体[EPR4847] - BSA and Azide free

Anti-GSC antibody [EPR4847] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GSC antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

Homeobox protein goosecoid, GSC

1 Images

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Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (AB247751)

This data was developed using ab109024, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GSC antibody [EPR4847] (<a href='/products/primary-antibodies/gsc-antibody-epr4847-ab109024'>ab109024</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

HT29 lysate at 10 µg

Lane 3:

MCF7 lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Predicted band size: 28 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-GSC antibody [EPR4847]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4847

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab247751 is the carrier-free version of ab109024.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The biological target GSC also known as Goosecoid is a transcription factor with a molecular weight of approximately 28 kDa. It is expressed during early embryonic development and plays an important role in mesoderm patterning and differentiation. GSC is primarily found in specific regions such as the Spemann organizer in amphibians and the primitive streak in other vertebrates. The transcriptional activity of GSC is regulated through interactions with other proteins and signaling pathways which modulate its expression in these tissues.

Biological function summary

Goosecoid functions as a homeobox-containing transcription factor that regulates gene expression critical for embryogenesis. It forms part of transcriptional complexes that are involved in modulating the activity of target genes essential for morphogenetic movements. GSC activates or represses specific genes to control the cell fate and tissue patterning during development. It coordinates the expression of genes required for establishing body axis formation reflecting its role in orchestrating developmental processes.

Pathways

Goosecoid interacts with the Wnt and TGF-beta signaling pathways which are vital for early embryonic development. These pathways regulate the expression of several proteins such as beta-catenin and Smad proteins which are important for transducing signals that affect cell differentiation and migration. GSC’s involvement in these pathways highlights its role as a mediator of signals necessary for the coordination of complex developmental processes.

Disruptions in Goosecoid's expression or function are linked to congenital malformations such as craniofacial disorders and skeletal anomalies. Aberrant regulation of GSC may result in improper development leading to conditions such as Robinow syndrome where facial abnormalities and short stature are observed. Additionally alterations in the activity of proteins like BMP4 which interact with GSC further highlight the significance of this target in developmental pathologies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring (By similarity). Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals.

See full target information GSC

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重组Anti-GSC抗体[EPR4847] - BSA and Azide free