Anti-GSAP抗体(ab106630)
Key features and details
- Rabbit polyclonal to GSAP
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-GSAP抗体 -
描述
兔多克隆抗体to GSAP -
宿主
Rabbit -
特异性
ab106630 is predicted to not cross-react with other F-box protein family members. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human GSAP (C terminal).
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阳性对照
- WB: EL4 cell lysate. IHC-P: Mouse brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab106630 was purified by affinity chromatography via a peptide column. -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 98 kDa.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 98 kDa. |
靶标
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功能
Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta-amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the GSAP family. -
翻译后修饰
The protein is first synthesized as a holoprotein form of 98 kDa and rapidly processed into the gamma-secretase-activating protein 16 kDa C-terminal form, which constitutes the predominant form. -
细胞定位
Golgi apparatus > trans-Golgi network. - Information by UniProt
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数据库链接
- Entrez Gene: 212167 Mouse
- SwissProt: Q3TCV3 Mouse
- Unigene: 121705 Mouse
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别名
- A530088I07Rik antibody
- DKFZp667B242 antibody
- Gamma-secretase-activating protein 16 kDa C-terminal form antibody
see all
图片
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Western blot - Anti-GSAP antibody (ab106630)All lanes : Anti-GSAP antibody (ab106630) at 0.25 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSAP antibody (ab106630)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining GSAP with ab106630 at 20µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSAP antibody (ab106630)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling GSAP with Anti-GSAP antibody (ab106630) at 5μg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab106630 被引用在 5 文献中.
- Angira D et al. To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides. ACS Omega 8:13435-13443 (2023). PubMed: 37065030
- Jin C et al. Modulation of amyloid precursor protein cleavage by γ-secretase activating protein through phase separation. Proc Natl Acad Sci U S A 119:e2122292119 (2022). PubMed: 35298330
- Dong J et al. Integrated analysis of genome-wide DNA methylation and cancer-associated fibroblasts identified prognostic biomarkers and immune checkpoint blockade in lower grade gliomas. Front Oncol 12:977251 (2022). PubMed: 36727078
- Hajjari SN et al. MicroRNA-4422-5p as a Negative Regulator of Amyloidogenic Secretases: A Potential Biomarker for Alzheimer's Disease. Neuroscience 463:108-115 (2021). PubMed: 33836245
- Wang L et al. PTH-induced EndMT via miR-29a-5p/GSAP/Notch1 pathway contributed to valvular calcification in rats with CKD. Cell Prolif 54:e13018 (2021). PubMed: 33945189