Anti-GRWD1抗体(ab176815)
Key features and details
- Rabbit polyclonal to GRWD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GRWD1抗体
参阅全部 GRWD1 一抗 -
描述
兔多克隆抗体to GRWD1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan -
免疫原
Synthetic peptide within Human GRWD1 aa 1-50. The exact sequence is proprietary. NP_113673.2
Sequence:MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD
Database link: Q9BQ67 -
阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176815于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 540739 Cow
- Entrez Gene: 83743 Human
- Omim: 610597 Human
- SwissProt: Q1JQD2 Cow
- SwissProt: Q9BQ67 Human
- Unigene: 400625 Human
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别名
- CDW4 antibody
- CUL4 and DDB1 associated WDR protein 4 antibody
- Glutamate rich WD repeat containing 1 antibody
see all
图片
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All lanes : Anti-GRWD1 antibody (ab176815) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds -
Detection of GRWD1 by Western Blot of Immunprecipitate.
ab176815 at 0.1 µg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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Datasheet download
文献 (0)
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