重组Anti-GRP94抗体[EPR22847-50] (ab238126)
![Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-384452-anti-grp94-antibody-epr22847-50-western-blot-hek293t-hela.jpg "Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22847-50] to GRP94
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-GRP94抗体[EPR22847-50]
参阅全部 GRP94 一抗 -
描述
兔单克隆抗体[EPR22847-50] to GRP94 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human kidney and stomach tissue lysates; mouse brain and placenta tissue lysates; rat brain, placenta and stomach tissue lysates; HeLa, MCF7, HEK293T, MEF, PC-12, C6 and NIH/3T3 whole cell lysates. IHC-P: Human kidney tissue; mouse and rat colon tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22847-50 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab238126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/1000.
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| IP |
1/30.
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| Flow Cyt (Intra) |
1/600.
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| 说明 |
|---|
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WB
1/1000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa). |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/1000. |
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IP
1/30. |
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Flow Cyt (Intra)
1/600. |
靶标
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功能
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
序列相似性
Belongs to the heat shock protein 90 family. -
细胞定位
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: Q66HD0 Rat
- Unigene: 192374 Human
see all -
别名
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
图片
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Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)All lanes : Anti-GRP94 antibody [EPR22847-50] (ab238126) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : HSP90B1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDaLanes 1-3: Merged signal (red and green). Green - ab238126 observed at 94 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab238126 Anti-GRP94 antibody [EPR22847-50] was shown to specifically react with GRP94 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266313 (knockout cell lysate ab257254) was used. Wild-type and GRP94 knockout samples were subjected to SDS-PAGE. ab238126 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-9-anti-grp94-antibody-epr22847-50-immunohistochemistry-colon-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GRP94 with ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat colon (PMID: 23572575) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-8-anti-grp94-antibody-epr22847-50-immunohistochemistry-colon-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GRP94 with ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse colon (PMID: 23572575) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-7-anti-grp94-antibody-epr22847-50-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] (ab238126)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling GRP94 with ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney (PMID: 20520781) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-6-anti-grp94-antibody-epr22847-50-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] (ab238126)GRP94 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab238126 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238126 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab238126 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238126 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds. -
![Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-5-anti-grp94-antibody-epr22847-50-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] (ab238126)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GRP94 with ab238126 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-4-anti-grp94-antibody-epr22847-50-western-blot-2.jpg)
Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)All lanes : Anti-GRP94 antibody [EPR22847-50] (ab238126) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse placenta tissue lysate at 20 µg
Lane 3 : Mouse stomach tissue lysate at 20 µg
Lane 4 : Rat brain tissue lysate at 20 µg
Lane 5 : Rat placenta tissue lysate at 20 µg
Lane 6 : Rat stomach tissue lysate at 20 µg
Lane 7 : C6 (rat glial tumor glial cell line) whole cell lysate at 20 µg
Lane 8 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 26 seconds; Lanes 2-5: 10 seconds; Lane 6: 92 seconds; Lanes 7-8: 6 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26858453, 29949743).
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![Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-3-anti-grp94-antibody-epr22847-50-western-blot-1.jpg)
Western blot - Anti-GRP94 antibody [EPR22847-50] (ab238126)All lanes : Anti-GRP94 antibody [EPR22847-50] (ab238126) at 1/1000 dilution
Lane 1 : Human brain tissue lysate at 20 µg
Lane 2 : Human kidney tissue lysate at 20 µg
Lane 3 : Human stomach tissue lysate at 20 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 6 : MEF (Mouse embryonic fibroblast (immortalized) cell line) whole cell lysate at 20 µg
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1 & 2: 10 seconds; Lane 3: 37 seconds; Lanes 4-6: 10 seconds; Lane 7: 26 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26858453, 29949743).
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-2-anti-grp94-antibody-epr22847-50-immunocytochemistry-nih-3t3-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] (ab238126)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling GRP94 with ab238126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing endoplasmic reticulum staining in NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). The endoplasmic reticulum is stained with Anti-KDEL antibody [10C3] (ab12223), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-1-anti-antibody-epr22847-50-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] (ab238126)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GRP94 with ab238126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing endoplasmic reticulum staining in HeLa cell line. The nuclear counter stain is DAPI (blue). The endoplasmic reticulum is stained with Anti-KDEL antibody [10C3] (ab12223), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-GRP94 antibody [EPR22847-50] (ab238126)](https://www.abcam.cn/ps/products/238/ab238126/Images/ab238126-11-benefits-of-recombinant-antibodies.png)
Anti-GRP94 antibody [EPR22847-50] (ab238126)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (18)
ab238126 被引用在 18 文献中.
- Wang Y et al. Senescence-related gene c-Myc affects bladder cancer cell senescence by interacting with HSP90B1 to regulate cisplatin sensitivity. Aging (Albany NY) 15:7408-7423 (2023). PubMed: 37433010
- Gorgun C et al. Preconditioned Mesenchymal Stromal Cell-Derived Extracellular Vesicles (EVs) Counteract Inflammaging. Cells 11:N/A (2022). PubMed: 36429124
- Vdovenko D et al. Microvesicles released from activated CD4+ T cells alter microvascular endothelial cell function. Eur J Clin Invest 52:e13769 (2022). PubMed: 35316536
- Cai J et al. SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35419615
- Zhang S & Jin Z Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis. Oxid Med Cell Longev 2022:5517648 (2022). PubMed: 35480871